702779
antibody from Invitrogen Antibodies
Targeting: SACS
ARSACS, DKFZp686B15167, DNAJC29, KIAA0730, PPP1R138, SPAX6
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 702779 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Sacsin DNAJ domain Antibody (4H18L14), ABfinity™ Rabbit Monoclonal
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with Monkey, Rabbit, Cat
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 4H18L14
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on different concentrations of recombinant Sacsin DNAJ domain protein. The blots were probed with ABfinity™ Anti-Sacsin DNAJ domain Recombinant Rabbit Monoclonal Antibody (Product # 702779, 2.5 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/ml, 1:4000 dilution). A 35 kDa band corresponding to Sacsin DNAJ domain was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, SH-SY5Y cells were fixed and permeabilized for detection of endogenous Sacsin DNAJ domain using ABfinity™ Anti- Sacsin DNAJ domain Recombinant Rabbit Monoclonal Antibody (Product# 702779, 5 µg/ml) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Sacsin DNAJ domain (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents MitoTracker® Red CMXRos (Product # M7512, 50nM). Panel d) is a composite image of Panels a, b and c clearly demonstrating partial mitochondrial overlap of Sacsin DNAJ domain. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.