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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- NBP2-42184 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal PCDHGA3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Geater then 75% identity with other Gamma-protocadherin-A proteinsDetects approx 100kDa. Cross-reacts with other Gamma protocadherin-A proteins. No cross-reactivity against Gamma protocadherin-B or -C proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCDHGA3 Antibody (S144-32) - Pan Specific [NBP2-42184] - analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 showing detection of 100 kDa Protocadherin Gamma A (pan) protein using Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody, Clone S144-32 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 100 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PCDHGA3 Antibody (S144-32) - Pan Specific [NBP2-42184] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.
