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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 666304 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#666304, RRID:AB_2632919
- Product name
- Purified anti-VGLUT3
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 2B9
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- -20°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Western blot of purified anti-VGLUT3 antibody (clone 2B9) and isotype-matched IgG1 control. Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain tissue lysate; Lane 3: 20 µg of rat brain tissue lysate; Lane 4: 20 µg of mouse brain tissue lysate; Lane 5: 20 µg of SH-SY5Y cell lysate. The blots were incubated with either the VGLUT3 or mouse IgG1 antibodies at 5 µg/mL overnight at 4°C, followed by incubation with an HRP labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
- Conjugate
- Unconjugated
