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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 12199-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP25 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LKNVGNTCW FSAVIQSLFN LLEFRRLVLN YKPPSNAQDL PRNQKEHRNL PFMRELRYLF ALLVGTKRKY VDPSRAVEIL KDAFKSNDSQ QEWHQDYRKF RETTMYLIIG LENFQRESYI DSLLFLICAY QNNKELLSKG LYRGHDEELI SHYRRECLLK LNEQAAELFE SGEDREVNNG LIIMNEFIVP FLPLLLVDEM EEKDILAVED MRNRWCSYLG QEMEPHLQEK LTDFLPKLLD CSMEIKSFHE PPKLPSYSTH ELCERFARIM LSLSRTPADG R (171-451 aa encoded by BC015930)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.17 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 12199-1-AP ( USP25 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12199-1-AP ( USP25 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12199-1-AP ( USP25 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-USP25 (IP: 12199-1-AP, 4 µg; Detection: 12199-1-AP 1:300) with RAW 264.7 cells lysate 1600 µg.
