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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Immunohistochemistry [3]
- Flow cytometry [2]
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- Product number
- NB100-1642 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1642, RRID:AB_10129871
- Product name
- Rat Monoclonal CD31/PECAM-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Progenitor cell combination normalizes retinal vascular development in the oxygen-induced retinopathy (OIR) model.
Mesenchymal stem cells expressing interleukin-18 inhibit breast cancer in a mouse model.
Host STING-dependent MDSC mobilization drives extrinsic radiation resistance.
Spinal RacGAP α-Chimaerin Is Required to Establish the Midline Barrier for Proper Corticospinal Axon Guidance.
Preexisting smooth muscle cells contribute to neointimal cell repopulation at an incidence varying widely among individual lesions.
Magnetic Resonance Q Mapping Reveals a Decrease in Microvessel Density in the arcAβ Mouse Model of Cerebral Amyloidosis.
Oseltamivir phosphate monotherapy ablates tumor neovascularization, growth, and metastasis in mouse model of human triple-negative breast adenocarcinoma.
In vivo imaging of hypoxia-inducible factor regulation in a subcutaneous and orthotopic GL261 glioma tumor model using a reporter gene assay.
Transcription factor Ets1 cooperates with estrogen receptor α to stimulate estradiol-dependent growth in breast cancer cells and tumors.
VE-cadherin regulates endothelial actin activating Rac and increasing membrane association of Tiam.
In vivo activities of mutants of vascular endothelial growth factor (VEGF) with differential in vitro activities.
Impact of the tumour bed effect on microenvironment, radiobiological hypoxia and the outcome of fractionated radiotherapy of human FaDu squamous-cell carcinoma growing in the nude mouse.
Development of endothelial cell lines from embryonic stem cells: A tool for studying genetically manipulated endothelial cells in vitro.
Leukocyte recruitment in the cerebrospinal fluid of mice with experimental meningitis is inhibited by an antibody to junctional adhesion molecule (JAM).
Vascular architecture and microenvironmental parameters in human squamous cell carcinoma xenografts: effects of carbogen and nicotinamide.
Vascular endothelial-cadherin is an important determinant of microvascular integrity in vivo.
Vascular density in melanoma xenografts correlates with vascular permeability factor expression but not with metastatic potential.
A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants.
A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants.
A role for FGF-6 in skeletal muscle regeneration.
Analysis of the tumor vasculature and metastatic behavior of xenografts of human melanoma cell lines transfected with vascular permeability factor.
Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium.
Li Calzi S, Shaw LC, Moldovan L, Shelley WC, Qi X, Racette L, Quigley JL, Fortmann SD, Boulton ME, Yoder MC, Grant MB
JCI insight 2019 Nov 1;4(21)
JCI insight 2019 Nov 1;4(21)
Mesenchymal stem cells expressing interleukin-18 inhibit breast cancer in a mouse model.
Liu X, Hu J, Li Y, Cao W, Wang Y, Ma Z, Li F
Oncology letters 2018 May;15(5):6265-6274
Oncology letters 2018 May;15(5):6265-6274
Host STING-dependent MDSC mobilization drives extrinsic radiation resistance.
Liang H, Deng L, Hou Y, Meng X, Huang X, Rao E, Zheng W, Mauceri H, Mack M, Xu M, Fu YX, Weichselbaum RR
Nature communications 2017 Nov 23;8(1):1736
Nature communications 2017 Nov 23;8(1):1736
Spinal RacGAP α-Chimaerin Is Required to Establish the Midline Barrier for Proper Corticospinal Axon Guidance.
Katori S, Noguchi-Katori Y, Itohara S, Iwasato T
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Aug 9;37(32):7682-7699
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Aug 9;37(32):7682-7699
Preexisting smooth muscle cells contribute to neointimal cell repopulation at an incidence varying widely among individual lesions.
Yang P, Hong MS, Fu C, Schmit BM, Su Y, Berceli SA, Jiang Z
Surgery 2016 Feb;159(2):602-12
Surgery 2016 Feb;159(2):602-12
Magnetic Resonance Q Mapping Reveals a Decrease in Microvessel Density in the arcAβ Mouse Model of Cerebral Amyloidosis.
Ielacqua GD, Schlegel F, Füchtemeier M, Xandry J, Rudin M, Klohs J
Frontiers in aging neuroscience 2015;7:241
Frontiers in aging neuroscience 2015;7:241
Oseltamivir phosphate monotherapy ablates tumor neovascularization, growth, and metastasis in mouse model of human triple-negative breast adenocarcinoma.
Haxho F, Allison S, Alghamdi F, Brodhagen L, Kuta VE, Abdulkhalek S, Neufeld RJ, Szewczuk MR
Breast cancer (Dove Medical Press) 2014;6:191-203
Breast cancer (Dove Medical Press) 2014;6:191-203
In vivo imaging of hypoxia-inducible factor regulation in a subcutaneous and orthotopic GL261 glioma tumor model using a reporter gene assay.
Bürgi S, Seuwen A, Keist R, Vom Berg J, Grandjean J, Rudin M
Molecular imaging 2014;13
Molecular imaging 2014;13
Transcription factor Ets1 cooperates with estrogen receptor α to stimulate estradiol-dependent growth in breast cancer cells and tumors.
Kalet BT, Anglin SR, Handschy A, O'Donoghue LE, Halsey C, Chubb L, Korch C, Duval DL
PloS one 2013;8(7):e68815
PloS one 2013;8(7):e68815
VE-cadherin regulates endothelial actin activating Rac and increasing membrane association of Tiam.
Lampugnani MG, Zanetti A, Breviario F, Balconi G, Orsenigo F, Corada M, Spagnuolo R, Betson M, Braga V, Dejana E
Molecular biology of the cell 2002 Apr;13(4):1175-89
Molecular biology of the cell 2002 Apr;13(4):1175-89
In vivo activities of mutants of vascular endothelial growth factor (VEGF) with differential in vitro activities.
Leenders W, van Altena M, Lubsen N, Ruiter D, De Waal R
International journal of cancer 2001 Feb 1;91(3):327-33
International journal of cancer 2001 Feb 1;91(3):327-33
Impact of the tumour bed effect on microenvironment, radiobiological hypoxia and the outcome of fractionated radiotherapy of human FaDu squamous-cell carcinoma growing in the nude mouse.
Zips D, Eicheler W, Brüchner K, Jackisch T, Geyer P, Petersen C, van der Kogel AJ, Baumann M
International journal of radiation biology 2001 Dec;77(12):1185-93
International journal of radiation biology 2001 Dec;77(12):1185-93
Development of endothelial cell lines from embryonic stem cells: A tool for studying genetically manipulated endothelial cells in vitro.
Balconi G, Spagnuolo R, Dejana E
Arteriosclerosis, thrombosis, and vascular biology 2000 Jun;20(6):1443-51
Arteriosclerosis, thrombosis, and vascular biology 2000 Jun;20(6):1443-51
Leukocyte recruitment in the cerebrospinal fluid of mice with experimental meningitis is inhibited by an antibody to junctional adhesion molecule (JAM).
Del Maschio A, De Luigi A, Martin-Padura I, Brockhaus M, Bartfai T, Fruscella P, Adorini L, Martino G, Furlan R, De Simoni MG, Dejana E
The Journal of experimental medicine 1999 Nov 1;190(9):1351-6
The Journal of experimental medicine 1999 Nov 1;190(9):1351-6
Vascular architecture and microenvironmental parameters in human squamous cell carcinoma xenografts: effects of carbogen and nicotinamide.
Bussink J, Kaanders JH, Rijken PF, Peters JP, Hodgkiss RJ, Marres HA, van der Kogel AJ
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 1999 Feb;50(2):173-84
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 1999 Feb;50(2):173-84
Vascular endothelial-cadherin is an important determinant of microvascular integrity in vivo.
Corada M, Mariotti M, Thurston G, Smith K, Kunkel R, Brockhaus M, Lampugnani MG, Martin-Padura I, Stoppacciaro A, Ruco L, McDonald DM, Ward PA, Dejana E
Proceedings of the National Academy of Sciences of the United States of America 1999 Aug 17;96(17):9815-20
Proceedings of the National Academy of Sciences of the United States of America 1999 Aug 17;96(17):9815-20
Vascular density in melanoma xenografts correlates with vascular permeability factor expression but not with metastatic potential.
Westphal JR, van't Hullenaar RG, van der Laak JA, Cornelissen IM, Schalkwijk LJ, van Muijen GN, Wesseling P, de Wilde PC, Ruiter DJ, de Waal RM
British journal of cancer 1997;76(5):561-70
British journal of cancer 1997;76(5):561-70
A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants.
Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A, Vecchi A
Arteriosclerosis, thrombosis, and vascular biology 1997 Aug;17(8):1599-604
Arteriosclerosis, thrombosis, and vascular biology 1997 Aug;17(8):1599-604
A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants.
Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A, Vecchi A
Arteriosclerosis, thrombosis, and vascular biology 1997 Aug;17(8):1599-604
Arteriosclerosis, thrombosis, and vascular biology 1997 Aug;17(8):1599-604
A role for FGF-6 in skeletal muscle regeneration.
Floss T, Arnold HH, Braun T
Genes & development 1997 Aug 15;11(16):2040-51
Genes & development 1997 Aug 15;11(16):2040-51
Analysis of the tumor vasculature and metastatic behavior of xenografts of human melanoma cell lines transfected with vascular permeability factor.
Pötgens AJ, van Altena MC, Lubsen NH, Ruiter DJ, de Waal RM
The American journal of pathology 1996 Apr;148(4):1203-17
The American journal of pathology 1996 Apr;148(4):1203-17
Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium.
Vecchi A, Garlanda C, Lampugnani MG, Resnati M, Matteucci C, Stoppacciaro A, Schnurch H, Risau W, Ruco L, Mantovani A
European journal of cell biology 1994 Apr;63(2):247-54
European journal of cell biology 1994 Apr;63(2):247-54
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) and PE-conjugated F4/80 for 2 hours at the room temperature. Image using the Alexa Fluor 647 format of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Immunohistochemistry for vessel staining (CD31/PECAM-1) and beta-amyloid comparing 13- and 24-month old non-transgenic littermates. Overviews (scale bars = 200 um) of the corpus callosum (CC), thalamus (TH), and cerebellum (CB). Insets show the regions with higher magnification (scale bar = 50 um). Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fnagi.2015.00241/abstract), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Immunohistochemistry for vessel staining (CD31) and beta-amyloid comparing 13- and 24-month old arcAbeta mice. Overviews (scale bars = 200 um) of the corpus callosum (CC), thalamus (TH), and cerebellum (CB). Insets show the regions with higher magnification (scale bar = 50 um). Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fnagi.2015.00241/abstract), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - A surface stain was performed on MS-1 Cells with CD31/PECAM-1 Antibody (MEC 7.46) NB100-1642(blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - CD31/PECAM-1 Antibody (MEC 7.46) was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
