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- References [22]
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- Product number
- BBA7 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD31/PECAM-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD31/PEACAM-1. In Western blots, no cross-reactivity with recombinant human (rh) E-Selectin, rhICAM-1, -2, -3, rhVCAM-1, or recombinant mouse VCAM-1 was observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 9G11
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate Into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain.
Straightforward method for singularized and region-specific CNS microvessels isolation.
Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes.
In Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling.
Endothelial Surface Protrusion by a Point Force.
Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis.
Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms.
The ACVR1 R206H mutation found in fibrodysplasia ossificans progressiva increases human induced pluripotent stem cell-derived endothelial cell formation and collagen production through BMP-mediated SMAD1/5/8 signaling.
Vascular adhesion protein-1 promotes liver inflammation and drives hepatic fibrosis.
Association between PON1 rs662 polymorphism and coronary artery disease.
Probing the biomechanical contribution of the endothelium to lymphocyte migration: diapedesis by the path of least resistance.
Linagliptin-mediated DPP-4 inhibition ameliorates kidney fibrosis in streptozotocin-induced diabetic mice by inhibiting endothelial-to-mesenchymal transition in a therapeutic regimen.
C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
Mechanisms of endothelial cell protection by blockade of the JAK2 pathway.
The role of endothelial cell attachment to elastic fibre molecules in the enhancement of monolayer formation and retention, and the inhibition of smooth muscle cell recruitment.
Disruption of graft endothelium correlates with early failure after off-pump coronary artery bypass surgery.
A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties.
Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties.
Identification of a novel transcript of human PECAM-1 and its role in the transendothelial migration of monocytes and Ca2+ mobilization.
Stimulation of primary human endothelial cell proliferation by IFN.
Stimulation of primary human endothelial cell proliferation by IFN.
Luzuriaga J, Pastor-Alonso O, Encinas JM, Unda F, Ibarretxe G, Pineda JR
Frontiers in physiology 2019;10:347
Frontiers in physiology 2019;10:347
Straightforward method for singularized and region-specific CNS microvessels isolation.
Dayton JR, Franke MC, Yuan Y, Cruz-Orengo L
Journal of neuroscience methods 2019 Apr 15;318:17-33
Journal of neuroscience methods 2019 Apr 15;318:17-33
Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes.
Ecklu-Mensah G, Olsen RW, Bengtsson A, Ofori MF, Hviid L, Jensen ATR, Adams Y
PloS one 2018;13(10):e0204177
PloS one 2018;13(10):e0204177
In Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling.
Yamamizu K, Iwasaki M, Takakubo H, Sakamoto T, Ikuno T, Miyoshi M, Kondo T, Nakao Y, Nakagawa M, Inoue H, Yamashita JK
Stem cell reports 2017 Mar 14;8(3):634-647
Stem cell reports 2017 Mar 14;8(3):634-647
Endothelial Surface Protrusion by a Point Force.
Chen Y, Lu L, Shao JY
Biophysical journal 2016 Mar 8;110(5):1150-7
Biophysical journal 2016 Mar 8;110(5):1150-7
Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis.
Srivastava SP, Shi S, Kanasaki M, Nagai T, Kitada M, He J, Nakamura Y, Ishigaki Y, Kanasaki K, Koya D
Scientific reports 2016 Jul 18;6:29884
Scientific reports 2016 Jul 18;6:29884
Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms.
Ebrahim NA, Leach L
Placenta 2016 Dec;48:87-98
Placenta 2016 Dec;48:87-98
The ACVR1 R206H mutation found in fibrodysplasia ossificans progressiva increases human induced pluripotent stem cell-derived endothelial cell formation and collagen production through BMP-mediated SMAD1/5/8 signaling.
Barruet E, Morales BM, Lwin W, White MP, Theodoris CV, Kim H, Urrutia A, Wong SA, Srivastava D, Hsiao EC
Stem cell research & therapy 2016 Aug 17;7(1):115
Stem cell research & therapy 2016 Aug 17;7(1):115
Vascular adhesion protein-1 promotes liver inflammation and drives hepatic fibrosis.
Weston CJ, Shepherd EL, Claridge LC, Rantakari P, Curbishley SM, Tomlinson JW, Hubscher SG, Reynolds GM, Aalto K, Anstee QM, Jalkanen S, Salmi M, Smith DJ, Day CP, Adams DH
The Journal of clinical investigation 2015 Feb;125(2):501-20
The Journal of clinical investigation 2015 Feb;125(2):501-20
Association between PON1 rs662 polymorphism and coronary artery disease.
Liu T, Zhang X, Zhang J, Liang Z, Cai W, Huang M, Yan C, Zhu Z, Han Y
European journal of clinical nutrition 2014 Sep;68(9):1029-35
European journal of clinical nutrition 2014 Sep;68(9):1029-35
Probing the biomechanical contribution of the endothelium to lymphocyte migration: diapedesis by the path of least resistance.
Martinelli R, Zeiger AS, Whitfield M, Sciuto TE, Dvorak A, Van Vliet KJ, Greenwood J, Carman CV
Journal of cell science 2014 Sep 1;127(Pt 17):3720-34
Journal of cell science 2014 Sep 1;127(Pt 17):3720-34
Linagliptin-mediated DPP-4 inhibition ameliorates kidney fibrosis in streptozotocin-induced diabetic mice by inhibiting endothelial-to-mesenchymal transition in a therapeutic regimen.
Kanasaki K, Shi S, Kanasaki M, He J, Nagai T, Nakamura Y, Ishigaki Y, Kitada M, Srivastava SP, Koya D
Diabetes 2014 Jun;63(6):2120-31
Diabetes 2014 Jun;63(6):2120-31
C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
Dentelli P, Rosso A, Balsamo A, Colmenares Benedetto S, Zeoli A, Pegoraro M, Camussi G, Pegoraro L, Brizzi MF
Blood 2007 May 15;109(10):4264-71
Blood 2007 May 15;109(10):4264-71
Mechanisms of endothelial cell protection by blockade of the JAK2 pathway.
Neria F, Caramelo C, Peinado H, González-Pacheco FR, Deudero JJ, de Solis AJ, Fernández-Sánchez R, Peñate S, Cano A, Castilla MA
American journal of physiology. Cell physiology 2007 Mar;292(3):C1123-31
American journal of physiology. Cell physiology 2007 Mar;292(3):C1123-31
The role of endothelial cell attachment to elastic fibre molecules in the enhancement of monolayer formation and retention, and the inhibition of smooth muscle cell recruitment.
Williamson MR, Shuttleworth A, Canfield AE, Black RA, Kielty CM
Biomaterials 2007 Dec;28(35):5307-18
Biomaterials 2007 Dec;28(35):5307-18
Disruption of graft endothelium correlates with early failure after off-pump coronary artery bypass surgery.
Manchio JV, Gu J, Romar L, Brown J, Gammie J, Pierson RN 3rd, Griffith B, Poston RS
The Annals of thoracic surgery 2005 Jun;79(6):1991-8
The Annals of thoracic surgery 2005 Jun;79(6):1991-8
A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
Carman CV, Springer TA
The Journal of cell biology 2004 Oct 25;167(2):377-88
The Journal of cell biology 2004 Oct 25;167(2):377-88
Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties.
Satchell SC, Anderson KL, Mathieson PW
Journal of the American Society of Nephrology : JASN 2004 Mar;15(3):566-74
Journal of the American Society of Nephrology : JASN 2004 Mar;15(3):566-74
Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties.
Satchell SC, Anderson KL, Mathieson PW
Journal of the American Society of Nephrology : JASN 2004 Mar;15(3):566-74
Journal of the American Society of Nephrology : JASN 2004 Mar;15(3):566-74
Identification of a novel transcript of human PECAM-1 and its role in the transendothelial migration of monocytes and Ca2+ mobilization.
Wei H, Song J, Fang L, Li G, Chatterjee S
Biochemical and biophysical research communications 2004 Aug 6;320(4):1228-35
Biochemical and biophysical research communications 2004 Aug 6;320(4):1228-35
Stimulation of primary human endothelial cell proliferation by IFN.
Gomez D, Reich NC
Journal of immunology (Baltimore, Md. : 1950) 2003 Jun 1;170(11):5373-81
Journal of immunology (Baltimore, Md. : 1950) 2003 Jun 1;170(11):5373-81
Stimulation of primary human endothelial cell proliferation by IFN.
Gomez D, Reich NC
Journal of immunology (Baltimore, Md. : 1950) 2003 Jun 1;170(11):5373-81
Journal of immunology (Baltimore, Md. : 1950) 2003 Jun 1;170(11):5373-81
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CD31/PECAM-1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD31/PECAM-1 in HUVEC Human Cells. CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
