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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA504268 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504268, RRID:AB_11124120
- Product name
- CST2 (Cystatin SA) mouse monoclonal antibody, clone OTI5C2 (formerly 5C2)
- Antibody type
- Monoclonal
- Description
- CST2 (Cystatin SA) mouse monoclonal antibody, clone OTI5C2 (formerly 5C2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CST2
- Isotype
- IgG
- Antibody clone number
- OTI5C2
- Vial size
- 100 µl
- Concentration
- 0.65 mg/ml
Submitted references Inhaled diesel exhaust alters the allergen-induced bronchial secretome in humans.
Mookherjee N, Piyadasa H, Ryu MH, Rider CF, Ezzati P, Spicer V, Carlsten C
The European respiratory journal 2018 Jan;51(1)
The European respiratory journal 2018 Jan;51(1)
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CST2 (RC209350, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CST2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 1 cell line by using anti-CST2 monoclonal antibody. (1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC209350 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CST2 antibody(TA504268), and then analyzed by flow cytometry.
- Validation comment
- FC
