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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- ARG55149 - Provider product page
- Provider
- Arigo
- Product name
- anti-AGAP4 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 498-531 (C-terminus) of Human AGAP4.
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of HT-1080 cell line stained with ARG55149 anti-AGAP4 antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells stained with ARG55149 anti-AGAP4 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded H. liver section stained with ARG55149 anti-AGAP4 antibody at 1:25 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded M. liver section stained with ARG55149 anti-AGAP4 antibody at 1:25 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: MCF-7 cells stained with ARG55149 anti-AGAP4 antibody (green) at 1:25 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
