PA5-70320

antibody from Invitrogen Antibodies

Targeting: TRMT9B C8orf79, FLJ36980, hTRM9L, KIAA1456, TRM9L

Provider product page for PA5-70320

Western blot

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Other assay [2]

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Product number: PA5-70320 - Provider product page
Provider: Invitrogen Antibodies
Product name: KIAA1456 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This target displays homology in the following species: Cow: 100%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

TRMT9B protein structure - PA5-70320 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Effect of KIAA1456 overexpression and depletion on mcm 5 U and mcm 5 s 2 U modification levels and on CHIKV infection. a Western blot showing KIAA1456 protein upregulation upon CHIKV infection. Values denote relative KIAA1456 expression normalized by tubulin expression calculated from 3 independent replicates and expressed as mean +- SEM. b Western blot showing KIAA1456 protein levels under plasmid-driven overexpression (KIAA1456-OE) and CHIKV infection conditions. Values represent relative KIAA1456 expression normalized by vinculin expression. A representative example from two independent replicates is shown. Note, that to be able to compare expression levels, 1:5 dilutions of the KIAA1456-OE overexpression samples were loaded. c Relative mcm 5 U and mcm 5 s 2 U modification levels in mock-infected cells, CHIKV-infected cells and in cells overexpressing KIAA1456 (KIAA1456-OE) determined by mass LC-MS-MS; bars represent the mean value +- SEM of 4 (in the case of mcm 5 after KIAA1456-OE) respectively 5 (in the remaining cases) independent replicates. p -values: mcm 5 - mock vs CHIKV: 0.011, mcm5 - mock vs KIAA-OE: 0.016. d Relative amount of CHIKV RNA levels ( p -value 0.0046) and ( e ) of CHIKV capsid protein levels in CHIKV-infected cells transfected with an empty plasmid or with a plasmid overexpressing KIAA1456 (KIAA1456-OE) determined by qPCR; bars represent the mean value +- SEM of 5 (empty) and 4 (KIAA-OE) independent replicates. CHIKV RNA and capsid protein expression

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Effect of DENV and HCV infection on KIAA1456 protein levels and mcm 5 and mcm 5 s 2 U levels. a Western blot showing KIAA1456 protein expression levels upon DENV and HCV infection. Values denote KIAA1456 expression normalized by tubulin expression relative to uninfected cells calculated from 3 independent replicates and expressed as mean +- SEM. b as in Fig. 5c but upon DENV and HCV infection. Bars represent the mean value +- SEM of 5 independent replicates ( p -value 0.007). Statistical analyses were performed using the two-tailed t -test. * p < 0.05.