BS7231

antibody from Bioworld Technology, Inc

Targeting: H3C4 H3/b, H3FB, HIST1H3D

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

BS7231

Provider

Bioworld Technology, Inc

Product name

Histone H3 (Di Methyl K4) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H3, di methylated at K4.

Description

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Bioworld Technology, Inc (provider)

Main image

Experimental details

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Histone H3 (Di-Methyl K4) antibody.