F48401

antibody from NSJ Bioreagents

Targeting: H3C4 H3/b, H3FB, HIST1H3D

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

F48401

Provider

NSJ Bioreagents

Product name

Phospho-Histone H3 Antibody (pS10)

Antibody type

Polyclonal

Antigen

This phospho-Histone H3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS10 of human HIST1H3A.

Description

Antigen affinity purified antibody

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the phospho-Histone H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from HeLa cell line, untreated or treated with Calyculin A (100ng/ml), using phospho-Histone H3 antibody

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from HeLa cell line, untreated or treated with FBS + Calyculin A (100ng/ml), using phospho-Histone H3 antibody

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from HeLa cell line using phospho-Histone H3 antibody at 1:1000.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of extracts from HeLa cells, untreated or treated with FBS + Calyculin A (100nM), using phospho-Histone H3 antibody (left) or nonphos Ab (right).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of extracts from HeLa cells, untreated or treated with FBS and Calyculin A (100nM), using phospho-Histone H3 antibody (left) or nonphos Ab (right).

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Dot blot analysis of phospho-Histone H3 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.