M12477-1

antibody from Boster Biological Technology

Targeting: H3C4 H3/b, H3FB, HIST1H3D

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

M12477-1

Provider

Boster Biological Technology

Product name

Anti-Methyl-Histone H3 (di K4) HIST1H3A Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J detection. Host: Rabbit.Size: 100ug/vial. Tested applications: ChIP, Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J information: Molecular Weight: 15404 MW; Subcellular Localization: Nucleus. Chromosome.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

AFF-8

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human liver, using Methyl-Histone H3 (di K4) Antibody(M12477-1)HIST1H3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (M12477-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image