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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- 17-615 - Provider product page
- Provider
- EMD Millipore
- Proper citation
- Millipore Cat#17-615, RRID:AB_1163437
- Product name
- ChIPAb+ Acetyl-Histone H3 - ChIP Validated Antibody and Primer Set
- Antibody type
- Polyclonal
- Antigen
- The acetyl-histone H3 antibody is made against a peptide corresponding to amino acids 1-20 of Tetrahymena histone H3 ARTKQTAR[K*]STGG[K*]APRKQL(C) where K* is acetylated)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 125 µg
- Storage
- Stable for 1 year at -20°C from date of receipt.
Submitted references Identification of STAT2 serine 287 as a novel regulatory phosphorylation site in type I interferon-induced cellular responses.
Lysine-specific demethylase 1 (LSD1) and histone deacetylase 1 (HDAC1) synergistically repress proinflammatory cytokines and classical complement pathway components.
Administration of vorinostat disrupts HIV-1 latency in patients on antiretroviral therapy.
Histone deacetylase complexes promote trinucleotide repeat expansions.
Microglial TIR-domain-containing adapter-inducing interferon-β (TRIF) deficiency promotes retinal ganglion cell survival and axon regeneration via nuclear factor-κB.
p53 mutants induce transcription of NF-κB2 in H1299 cells through CBP and STAT binding on the NF-κB2 promoter and gain of function activity.
Dynamics of plant histone modifications in response to DNA damage.
RNA polymerase II stalling promotes nucleosome occlusion and pTEFb recruitment to drive immortalization by Epstein-Barr virus.
Expression of sphingosine kinase 1 in amoeboid microglial cells in the corpus callosum of postnatal rats.
Epstein-Barr virus nuclear antigens 3C and 3A maintain lymphoblastoid cell growth by repressing p16INK4A and p14ARF expression.
Regulation of cyclin B2 expression and cell cycle G2/m transition by menin.
Acetylation of EKLF is essential for epigenetic modification and transcriptional activation of the beta-globin locus.
Steen HC, Nogusa S, Thapa RJ, Basagoudanavar SH, Gill AL, Merali S, Barrero CA, Balachandran S, Gamero AM
The Journal of biological chemistry 2013 Jan 4;288(1):747-58
The Journal of biological chemistry 2013 Jan 4;288(1):747-58
Lysine-specific demethylase 1 (LSD1) and histone deacetylase 1 (HDAC1) synergistically repress proinflammatory cytokines and classical complement pathway components.
Janzer A, Lim S, Fronhoffs F, Niazy N, Buettner R, Kirfel J
Biochemical and biophysical research communications 2012 May 18;421(4):665-70
Biochemical and biophysical research communications 2012 May 18;421(4):665-70
Administration of vorinostat disrupts HIV-1 latency in patients on antiretroviral therapy.
Archin NM, Liberty AL, Kashuba AD, Choudhary SK, Kuruc JD, Crooks AM, Parker DC, Anderson EM, Kearney MF, Strain MC, Richman DD, Hudgens MG, Bosch RJ, Coffin JM, Eron JJ, Hazuda DJ, Margolis DM
Nature 2012 Jul 25;487(7408):482-5
Nature 2012 Jul 25;487(7408):482-5
Histone deacetylase complexes promote trinucleotide repeat expansions.
Debacker K, Frizzell A, Gleeson O, Kirkham-McCarthy L, Mertz T, Lahue RS
PLoS biology 2012 Feb;10(2):e1001257
PLoS biology 2012 Feb;10(2):e1001257
Microglial TIR-domain-containing adapter-inducing interferon-β (TRIF) deficiency promotes retinal ganglion cell survival and axon regeneration via nuclear factor-κB.
Lin S, Liang Y, Zhang J, Bian C, Zhou H, Guo Q, Xiong Y, Li S, Su B
Journal of neuroinflammation 2012 Feb 24;9:39
Journal of neuroinflammation 2012 Feb 24;9:39
p53 mutants induce transcription of NF-κB2 in H1299 cells through CBP and STAT binding on the NF-κB2 promoter and gain of function activity.
Vaughan CA, Singh S, Windle B, Sankala HM, Graves PR, Andrew Yeudall W, Deb SP, Deb S
Archives of biochemistry and biophysics 2012 Feb 1;518(1):79-88
Archives of biochemistry and biophysics 2012 Feb 1;518(1):79-88
Dynamics of plant histone modifications in response to DNA damage.
Drury GE, Dowle AA, Ashford DA, Waterworth WM, Thomas J, West CE
The Biochemical journal 2012 Aug 1;445(3):393-401
The Biochemical journal 2012 Aug 1;445(3):393-401
RNA polymerase II stalling promotes nucleosome occlusion and pTEFb recruitment to drive immortalization by Epstein-Barr virus.
Palermo RD, Webb HM, West MJ
PLoS pathogens 2011 Oct;7(10):e1002334
PLoS pathogens 2011 Oct;7(10):e1002334
Expression of sphingosine kinase 1 in amoeboid microglial cells in the corpus callosum of postnatal rats.
Lin H, Baby N, Lu J, Kaur C, Zhang C, Xu J, Ling EA, Dheen ST
Journal of neuroinflammation 2011 Feb 11;8:13
Journal of neuroinflammation 2011 Feb 11;8:13
Epstein-Barr virus nuclear antigens 3C and 3A maintain lymphoblastoid cell growth by repressing p16INK4A and p14ARF expression.
Maruo S, Zhao B, Johannsen E, Kieff E, Zou J, Takada K
Proceedings of the National Academy of Sciences of the United States of America 2011 Feb 1;108(5):1919-24
Proceedings of the National Academy of Sciences of the United States of America 2011 Feb 1;108(5):1919-24
Regulation of cyclin B2 expression and cell cycle G2/m transition by menin.
Wu T, Zhang X, Huang X, Yang Y, Hua X
The Journal of biological chemistry 2010 Jun 11;285(24):18291-300
The Journal of biological chemistry 2010 Jun 11;285(24):18291-300
Acetylation of EKLF is essential for epigenetic modification and transcriptional activation of the beta-globin locus.
Sengupta T, Chen K, Milot E, Bieker JJ
Molecular and cellular biology 2008 Oct;28(20):6160-70
Molecular and cellular biology 2008 Oct;28(20):6160-70
No comments: Submit comment
Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- 0.01-0.05 µg/mL of this lot detected acetylated histone H3 in acid extracted proteins from HeLa cells. Sodium butyrate, an inhibitor of histone deacetylases, was used to enhance detection of acetylated histone H3. A previous lot detected acetylated histone H3 in Tetrahymena macronuclei. Higher concentrations of this lot also detected unacetylated histone H3.
- Validation comment
- WB
Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- Sonicated chromatin prepared from untreated HeLa cells (1 X 106 cell equivalents) was subjected to chromatin immunoprecipitation using 5 µg of either a normal rabbit IgG or Anti-Acetyl Histone H3 and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of acetyl histone H3 associated DNA fragments was verified by qPCR using control ChIP Primers, flanking the human GAPDH promoter.
- Validation comment
- ChIP
