ALX-804-673-C050

antibody from Enzo Life Sciences

Targeting: H3C4 H3/b, H3FB, HIST1H3D

Western blot

ELISA

Chromatin Immunoprecipitation

Antibody data

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Validation data

H3C4 protein structure - ALX-804-673-C050 shown in red.

Supportive validation

Submitted by: Enzo Life Sciences (provider)
Main image
Experimental details: Western blot analysis of HeLa histones using MAb toÊHistone H3 (K9 methylated) (6F12-H4) (Prod. No. ALX-804-673).Lane 1: Fibercell supernatant (1:500)Lane 2: Purified antibody (ALX-804-673) (1:1'000)Lane 3: Purified antibody (ALX-804-673) (1:5'000)~1µg of HeLa cell total histones per lane, 10s exposure.

Supportive validation

Submitted by: Enzo Life Sciences (provider)
Main image
Experimental details: Peptide competition assay using MAb toÊHistone H3 (K9 methylated) (6F12-H4) (Prod. No. ALX-804-673). Method: To determine the specificity of MAb toÊHistone H3 (K9 methylated) (6F12-H4), the antibody was diluted 1:200 in 0.5% NFDM in PBS-T and incubated for 6 hours at 4¡C with a molar excess of peptides corresponding to aa 1-20 of human histone H3, containing either unmodified K9 (lanes 2-6), dimethylated K9 (lanes 8-12), or tri-methylated K9 (lanes 14-18). In each case, the molar excess of peptide over antibody was 100 fold, 50 fold, 20 fold, 10 fold, and 5 fold, respectively, as indicated by the arrowheads (from left to right) above the blot. The membrane containing histones prepared from HeLa cells was then incubated o/n at 4¡C with the antibody/peptide solutions, and processed for Western blotting. As control, one aliquot of antibody was incubated with water only (lane 1). Lanes 7 and 13 were left empty.

Supportive validation

Submitted by: Enzo Life Sciences (provider)
Main image
Experimental details: Immunocytochemistry of irradiated mouse embryonic fibroblasts (iMEFs) stained with DAPI and MAb to Histone H3 (K9 methylated) (6F12-H4) (Prod. No. ALX-804-673).Figure courtesy of Susanne Opravil and Thomas Jenuwein, Institute of Molecular Pathology (IMP) Vienna, Austria

Supportive validation

Submitted by: Enzo Life Sciences (provider)
Main image
Experimental details: ChIP analysis of known chromosomal Suv39h targets (H2K9me3 in major satellites, mouse ES cells) using MAb toÊHistone H3 (K9 methylated) (6F12-H4) (Prod. No. ALX-804-673).Figure courtesy of Inti Alberto De La Rosa Velazquez and Thomas Jenuwein, Institute of Molecular Pathology (IMP) Vienna, Austria.

Supportive validation

Submitted by: Enzo Life Sciences (provider)
Main image
Experimental details: Dot-blot analysisÊusing MAb to Histone H3 (K9 methylated) (6F12-H4) (Prod. No. ALX-804-673).Figure courtesy of Susanne Opravil and Thomas Jenuwein, Institute of Molecular Pathology (IMP) Vienna, Austria.