LS-C186089

antibody from LSBio

Targeting: TUBB8 bA631M21.2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C186089

Provider

LSBio

Product name

TUBB8 / Tubulin Beta 8 Antibody (aa366-444) LS-C186089

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

beta Tubulin 8 antibody immunoprecipitates beta Tubulin 8 protein in IP experiments. IP Sample:293T whole cell lysate/extract. A. 40 ug 293T whole cell lysate/extract. B. Control with 2 ug of preimmune rabbit IgG. C. Immunoprecipitation of beta Tubulin 8 protein by 2 ug of beta Tubulin 8 antibody . 10% SDS-PAGE. The immunoprecipitated beta Tubulin 8 protein was detected by beta Tubulin 8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

beta Tubulin 8 antibody immunoprecipitates beta Tubulin 8 protein in IP experiments. IP Sample:293T whole cell lysate/extract. A. 40 ug 293T whole cell lysate/extract. B. Control with 2 ug of preimmune rabbit IgG. C. Immunoprecipitation of beta Tubulin 8 protein by 2 ug of beta Tubulin 8 antibody . 10% SDS-PAGE. The immunoprecipitated beta Tubulin 8 protein was detected by beta Tubulin 8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

beta Tubulin 8 antibody [C2C3], C-term detects RP11-631M21.2 protein at cytosol on Mahlarvu xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Mahlarvu xenograft. beta Tubulin 8 antibody [C2C3], C-term dilution:1:500.

Blocking/Neutralizing

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Sample (30 ug whole cell lysate). A: A431, B: H1299. 10% SDS PAGE. TUBB8 antibody diluted at 1:1000

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

beta Tubulin 8 antibody [C2C3], C-term detects RP11-631M21.2 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract. 10 % SDS-PAGE. beta Tubulin 8 antibody [C2C3], C-term dilution:1:1000