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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- TA309065 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal antibody to beta Tubulin 8
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal antibody to beta Tubulin 8
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- TUBB8
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 1mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- beta Tubulin 8 antibody [C2C3], C-term detects RP11-631M21.2 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysa
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample(30 ug whole cell lysate). A:A431. B:H1299. 10% SDS PAGE. TA309065 diluted at 1:1000
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using beta Tubulin 8(TA309065) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- beta Tubulin 8 antibody immunoprecipitates beta Tubulin 8 protein in IP experiments. IP Sample: 293T whole cell lysate/extract. A. 40 ug 293T whole cell lysate/extract. B. Control with 2 ug of preimmune rabbit IgG. C. Immunoprecipitation of beta Tubulin 8
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- beta Tubulin 8 antibody [C2C3], C-term detects RP11-631M21.2 protein at cytosol on Mahlarvu xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Mahlarvu xenograft. beta Tubulin 8 antibody [C2C3], C-term (TA309065) dilution: 1:500.
- Validation comment
- IHC
