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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10200 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10200, RRID:AB_1671813
- Product name
- DLL4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of DLL4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Expression pattern and function of Notch2 in different subtypes of first trimester cytotrophoblast.
Membrane-bound delta-4 notch ligand reduces the proliferative activity of primitive human hematopoietic CD34+CD38low cells while maintaining their LTC-IC potential.
Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines.
Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis.
Plessl K, Haider S, Fiala C, Pollheimer J, Knöfler M
Placenta 2015 Apr;36(4):365-71
Placenta 2015 Apr;36(4):365-71
Membrane-bound delta-4 notch ligand reduces the proliferative activity of primitive human hematopoietic CD34+CD38low cells while maintaining their LTC-IC potential.
Lauret E, Catelain C, Titeux M, Poirault S, Dando JS, Dorsch M, Villeval JL, Groseil A, Vainchenker W, Sainteny F, Bennaceur-Griscelli A
Leukemia 2004 Apr;18(4):788-97
Leukemia 2004 Apr;18(4):788-97
Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines.
Tohda S, Murata-Ohsawa M, Sakano S, Nara N
International journal of oncology 2003 May;22(5):1073-9
International journal of oncology 2003 May;22(5):1073-9
Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis.
Liu ZJ, Shirakawa T, Li Y, Soma A, Oka M, Dotto GP, Fairman RM, Velazquez OC, Herlyn M
Molecular and cellular biology 2003 Jan;23(1):14-25
Molecular and cellular biology 2003 Jan;23(1):14-25
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using DLL4 polyclonal antibody (Cat # PAB10200) shows detection of a 74 KDa band corresponding to DLL4 in a lysate prepared from mouse pancreatic tissue.Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of DLL4 polyclonal antibody.Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.A chemiluminescence system was used for signal detection using a 3 min exposure time.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of DLL4 polyclonal antibody (Cat # PAB10200) was used at 20 ug/mL to detect DLL4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus.In contrast to reported findings, no staining was observed in vascular tissue.This image shows DLL4 staining of human ovary.Tissue was formalin-fixed and paraffin embedded.Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
