NB600-892

Shao L, Sottoriva K, Palasiewicz K, Zhang J, Hyun J, Soni SS, Paik NY, Gao X, Cuervo H, Malik AB, Rehman J, Lucas D, Pajcini KV
Haematologica 2019 Nov;104(11):2164-2177

Triggering of a Dll4-Notch1 loop impairs wound healing in diabetes.

Zheng X, Narayanan S, Sunkari VG, Eliasson S, Botusan IR, Grünler J, Catrina AI, Radtke F, Xu C, Zhao A, Ekberg NR, Lendahl U, Catrina SB
Proceedings of the National Academy of Sciences of the United States of America 2019 Apr 2;116(14):6985-6994

Maintenance of Bone Homeostasis by DLL1-Mediated Notch Signaling.

Muguruma Y, Hozumi K, Warita H, Yahata T, Uno T, Ito M, Ando K
Journal of cellular physiology 2017 Sep;232(9):2569-2580

Lymphatic deletion of calcitonin receptor-like receptor exacerbates intestinal inflammation.

Davis RB, Kechele DO, Blakeney ES, Pawlak JB, Caron KM
JCI insight 2017 Mar 23;2(6):e92465

A pilot study on plasma levels of micro-RNAs involved in angiogenesis and vascular maturation in patients with breast cancer.

Kontomanolis E, Mitrakas A, Giatromanolaki A, Kareli D, Panteliadou M, Pouliliou S, Koukourakis MI
Medical oncology (Northwood, London, England) 2017 Feb;34(2):20

Delta-like ligand 4 (DLL4) in the plasma and neoplastic tissues from breast cancer patients: correlation with metastasis.

Kontomanolis E, Panteliadou M, Giatromanolaki A, Pouliliou S, Efremidou E, Limberis V, Galazios G, Sivridis E, Koukourakis MI
Medical oncology (Northwood, London, England) 2014 May;31(5):945

CD34(-) cells at the apex of the human hematopoietic stem cell hierarchy have distinctive cellular and molecular signatures.

Anjos-Afonso F, Currie E, Palmer HG, Foster KE, Taussig DC, Bonnet D
Cell stem cell 2013 Aug 1;13(2):161-74

Tangential migration and proliferation of intermediate progenitors of GABAergic neurons in the mouse telencephalon.

Wu S, Esumi S, Watanabe K, Chen J, Nakamura KC, Nakamura K, Kometani K, Minato N, Yanagawa Y, Akashi K, Sakimura K, Kaneko T, Tamamaki N
Development (Cambridge, England) 2011 Jun;138(12):2499-509

Effect of Notch activation on the regenerative response to acute renal failure.

Gupta S, Li S, Abedin MJ, Wang L, Schneider E, Najafian B, Rosenberg M
American journal of physiology. Renal physiology 2010 Jan;298(1):F209-15

Suppressor of cytokine signaling 3 promotes bone marrow cells to differentiate into CD8+ T lymphocytes in lung tissue via up-regulating Notch1 expression.

Zhang Z, Zeng B, Zhang Z, Jiao G, Li H, Jing Z, Ouyang J, Yuan X, Chai L, Che Y, Zhang Y, Yang R
Cancer research 2009 Feb 15;69(4):1578-86

Cross-talk between tumor and endothelial cells involving the Notch3-Dll4 interaction marks escape from tumor dormancy.

Indraccolo S, Minuzzo S, Masiero M, Pusceddu I, Persano L, Moserle L, Reboldi A, Favaro E, Mecarozzi M, Di Mario G, Screpanti I, Ponzoni M, Doglioni C, Amadori A
Cancer research 2009 Feb 15;69(4):1314-23

Ectopic T-bet expression licenses dendritic cells for IL-12-independent priming of type 1 T cells in vitro.

Lipscomb MW, Chen L, Taylor JL, Goldbach C, Watkins SC, Kalinski P, Butterfield LH, Wesa AK, Storkus WJ
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 1;183(11):7250-8

Molecular profile of endothelial invasion of three-dimensional collagen matrices: insights into angiogenic sprout induction in wound healing.

Su SC, Mendoza EA, Kwak HI, Bayless KJ
American journal of physiology. Cell physiology 2008 Nov;295(5):C1215-29

Membrane-bound delta-4 notch ligand reduces the proliferative activity of primitive human hematopoietic CD34+CD38low cells while maintaining their LTC-IC potential.

Lauret E, Catelain C, Titeux M, Poirault S, Dando JS, Dorsch M, Villeval JL, Groseil A, Vainchenker W, Sainteny F, Bennaceur-Griscelli A
Leukemia 2004 Apr;18(4):788-97

Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines.

Tohda S, Murata-Ohsawa M, Sakano S, Nara N
International journal of oncology 2003 May;22(5):1073-9

Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis.

Liu ZJ, Shirakawa T, Li Y, Soma A, Oka M, Dotto GP, Fairman RM, Velazquez OC, Herlyn M
Molecular and cellular biology 2003 Jan;23(1):14-25

Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis.

Liu ZJ, Shirakawa T, Li Y, Soma A, Oka M, Dotto GP, Fairman RM, Velazquez OC, Herlyn M
Molecular and cellular biology 2003 Jan;23(1):14-25

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DLL4 Antibody [NB600-892] - Shows detection of a 74-kDa band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Delta-4 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: DLL4 Antibody [NB600-892] - This antibody was used at 20ug/mL in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary.