AP0243

antibody from Bioworld Technology, Inc

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AP0243

Provider

Bioworld Technology, Inc

Proper citation

Bioworld Technology Cat#AP0243, RRID:AB_2797464

Product name

HSP27 (phospho S78) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Serine 78.

Description

Hsp27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Bioworld Technology, Inc (provider)

Main image

Experimental details

Western blot (WB) analysis of p-HSP27 (S78) pAb at 1:500 dilutionLane1:Hela whole cell lysate treated with UV(4h)Lane2:NIH-3T3 whole cell lysate treated with UV(4h)Lane3:PC12 whole cell lysate treated with UV(4h)