M01819-1

antibody from Boster Biological Technology

Targeting: FOXO4 AFX1, MLLT7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M01819-1

Provider

Boster Biological Technology

Product name

Anti-FoxO4/Afx Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Anti-FoxO4 Rabbit Monoclonal Antibody tested for Flow Cytometry, IP, IF, ICC, WB in Human, Mouse, Rat

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

HEH-6

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of FoxO4 expression in human heart lysate (M01819-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO4 monoclonal antibody (Catalog # M01819-1) overnight at 4

Additional image