Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 11965-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EMG1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAAPSDGFK PRERSGGEQA QDWDALPPKR PRLGAGNKIG GRRLIVVLEG ASLETVKVGK TYELLNCDKH KSILLKNGRD PGEARPDITH QSLLMLMDSP LNRAGLLQVY IHTQKNVLIE VNPQTRIPRT FDRFCGLMVQ LLHKLSVRAA DGPQKLLKVI KNPVSDHFPV GCMKVGTSFS IPVVSDVREL VPSSDPIVFV VGAFAHGKVS VEYTEKMVSI SNYPLSAALT CAKLTTAFEE VWGVI (1-244 aa encoded by BC055314)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 11965-1-AP (EMG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-EMG1 (IP:11965-1-AP, 4ug; Detection:11965-1-AP 1:400) with HeLa cells lysate 1080ug.