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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [3]
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- Product number
- PA5-13416 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nurr1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine and rat based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400 µL
- Concentration
- 2.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Characterization and Study of Gene Expression Profiles of Human Periodontal Mesenchymal Stem Cells in Spheroid Cultures by Transcriptome Analysis.
Age-dependent decrease of Nurr1 protein expression in the gerbil hippocampus.
Suga T, Usui M, Onizuka S, Sano K, Sato T, Nakazawa K, Ariyoshi W, Nishihara T, Nakashima K
Stem cells international 2021;2021:5592804
Stem cells international 2021;2021:5592804
Age-dependent decrease of Nurr1 protein expression in the gerbil hippocampus.
Ahn JH, Lee JS, Cho JH, Park JH, Lee TK, Song M, Kim H, Kang SH, Won MH, Lee CH
Biomedical reports 2018 Jun;8(6):517-522
Biomedical reports 2018 Jun;8(6):517-522
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse brain tissue lysate using a NURR1 polyclonal antibody (Product # PA5-13416).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a NURR1 polyclonal antibody (Product # PA5-13416) in HeLa cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a NURR1 polyclonal antibody (Product # PA5-13416) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue using a NURR1 polyclonal antibody (Product # PA5-13416), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 1. Western blot analysis of Nurr1 protein expression in hippocampi derived from young, adult and aged gerbils. The RODs of immunoblot bands are shown as percentage values. Data are presented as the means +- standard error of the mean. *P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. Nurr1 immunohistochemistry in hippocampal regions of young, adult and aged gerbils. Nurr1 expression was detected in the (A-C) CA1 and (D-F) CA3 regions and (G-I) DG. In the young group, strong Nurr1 immunoreactivity was detected in pyramidal neurons of the SP in the CA1 and 3 regions (arrows) and in granule cells of the GCL in the DG (arrowheads). Nurr1 immunoreactivity in the SP and GCL gradually decreased in the adult and aged groups. Magnification, x20; scale bar, 100 um. Nurr1, nuclear receptor related-1 protein; DG, dentate gyrus; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum; ML, molecular layer; GCL, granule cell layer; PL, polymorphic layer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Validation of NR4A2 expression in spheroid cultures of hPDLMSC. (a) Expression of NR4A2 mRNA in monolayer and spheroid-cultured hPDLMSC on day 3 by real-time RT-PCR. * p < 0.05 (compared with monolayer culture). (b) Expression of NR4A2 protein in monolayer and spheroid-cultured hPDLMSC on day 3 by western blotting. (c) NR4A1 and NR4A3 mRNA expression in monolayer and spheroid-cultured hPDLMSC on day 3 by real-time RT-PCR. * p < 0.05 (compared with monolayer culture). All experiments were performed with samples from four donors.
