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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX107897 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107897, RRID:AB_1950048
- Product name
- Casein Kinase 2 alpha antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Modulation of Heat Shock Factor 1 Activity through Silencing of Ser303/Ser307 Phosphorylation Supports a Metabolic Program Leading to Age-Related Obesity and Insulin Resistance.
WITHDRAWN: Abrogation of heat shock factor 1 (Hsf1) phosphorylation deregulates its activity and lowers activation threshold, leading to obesity in mice.
Nrf2/ARE Pathway Involved in Oxidative Stress Induced by Paraquat in Human Neural Progenitor Cells.
Jin X, Qiao A, Moskophidis D, Mivechi NF
Molecular and cellular biology 2018 Sep 15;38(18)
Molecular and cellular biology 2018 Sep 15;38(18)
WITHDRAWN: Abrogation of heat shock factor 1 (Hsf1) phosphorylation deregulates its activity and lowers activation threshold, leading to obesity in mice.
Jin X, Qiao A, Moskophidis D, Mivechi NF
The Journal of biological chemistry 2017 Jul 19;
The Journal of biological chemistry 2017 Jul 19;
Nrf2/ARE Pathway Involved in Oxidative Stress Induced by Paraquat in Human Neural Progenitor Cells.
Dou T, Yan M, Wang X, Lu W, Zhao L, Lou D, Wu C, Chang X, Zhou Z
Oxidative medicine and cellular longevity 2016;2016:8923860
Oxidative medicine and cellular longevity 2016;2016:8923860
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Casein Kinase 2 alpha antibody detects CSNK2A1 protein by Western blot analysis.A. 50 μg mouse brain lysate/extract10 % SDS-PAGECasein Kinase 2 alpha antibody (GTX107897) dilution: 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30μg whole cell lysate)A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: Hep G2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908)10% SDS PAGEGTX107897 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Casein Kinase 2 alpha antibody detects CSNK2A1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CSNK2A1 protein stained by Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Casein Kinase 2 alpha 1(GTX107897) antibody at 1:100 dilution.
