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LearnMAB7578
antibody from R&D Systems
Targeting: NLRP3
AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCU, MWS, NALP3, PYPAF1
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- MAB7578 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse NLRP3/NALP3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse NLRP3/NALP3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human NLRP3/NALP3 is observed.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q8R4B8- Isotype
- IgG
- Antibody clone number
- 768319
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Endothelial GPR124 Exaggerates the Pathogenesis of Atherosclerosis by Activating Inflammation.
Eruca sativa seed extract: A novel natural product able to counteract neuroinflammation.
Downregulation of the NLRP3 inflammasome by adiponectin rescues Duchenne muscular dystrophy.
Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration.
The Antimalarial Chloroquine Suppresses LPS-Induced NLRP3 Inflammasome Activation and Confers Protection against Murine Endotoxic Shock.
Expression of TLR2, NOD1, and NOD2 and the NLRP3 Inflammasome in Renal Tubular Epithelial Cells of Male versus Female Mice.
Inhibition of NLRP3 Inflammasome Pathway by Butyrate Improves Corneal Wound Healing in Corneal Alkali Burn.
NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma.
Intestinal fungi contribute to development of alcoholic liver disease.
Knockout of Ccr2 alleviates photoreceptor cell death in rodent retina exposed to chronic blue light.
Suppression of NLRP3 inflammasome by γ-tocotrienol ameliorates type 2 diabetes.
Chinese Herbal Preparation Xuebijing Potently Inhibits Inflammasome Activation in Hepatocytes and Ameliorates Mouse Liver Ischemia-Reperfusion Injury.
The cyclopentenone prostaglandin 15d-PGJ2 inhibits the NLRP1 and NLRP3 inflammasomes.
Caspase-8 as an Effector and Regulator of NLRP3 Inflammasome Signaling.
ASC in renal collecting duct epithelial cells contributes to inflammation and injury after unilateral ureteral obstruction.
Gong DM, Zhang YL, Chen DY, Hong LJ, Han F, Liu QB, Jiang JJ, Lu YM
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(2):547-557
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(2):547-557
Eruca sativa seed extract: A novel natural product able to counteract neuroinflammation.
Gugliandolo A, Giacoppo S, Ficicchia M, Aliquò A, Bramanti P, Mazzon E
Molecular medicine reports 2018 May;17(5):6235-6244
Molecular medicine reports 2018 May;17(5):6235-6244
Downregulation of the NLRP3 inflammasome by adiponectin rescues Duchenne muscular dystrophy.
Boursereau R, Abou-Samra M, Lecompte S, Noel L, Brichard SM
BMC biology 2018 Mar 20;16(1):33
BMC biology 2018 Mar 20;16(1):33
Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration.
Kosmidou C, Efstathiou NE, Hoang MV, Notomi S, Konstantinou EK, Hirano M, Takahashi K, Maidana DE, Tsoka P, Young L, Gragoudas ES, Olsen TW, Morizane Y, Miller JW, Vavvas DG
Scientific reports 2018 Jan 11;8(1):461
Scientific reports 2018 Jan 11;8(1):461
The Antimalarial Chloroquine Suppresses LPS-Induced NLRP3 Inflammasome Activation and Confers Protection against Murine Endotoxic Shock.
Chen X, Wang N, Zhu Y, Lu Y, Liu X, Zheng J
Mediators of inflammation 2017;2017:6543237
Mediators of inflammation 2017;2017:6543237
Expression of TLR2, NOD1, and NOD2 and the NLRP3 Inflammasome in Renal Tubular Epithelial Cells of Male versus Female Mice.
DeWolf SE, Shigeoka AA, Scheinok A, Kasimsetty SG, Welch AK, McKay DB
Nephron 2017;137(1):68-76
Nephron 2017;137(1):68-76
Inhibition of NLRP3 Inflammasome Pathway by Butyrate Improves Corneal Wound Healing in Corneal Alkali Burn.
Bian F, Xiao Y, Zaheer M, Volpe EA, Pflugfelder SC, Li DQ, de Paiva CS
International journal of molecular sciences 2017 Mar 5;18(3)
International journal of molecular sciences 2017 Mar 5;18(3)
NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma.
Daley D, Mani VR, Mohan N, Akkad N, Pandian GSDB, Savadkar S, Lee KB, Torres-Hernandez A, Aykut B, Diskin B, Wang W, Farooq MS, Mahmud AI, Werba G, Morales EJ, Lall S, Wadowski BJ, Rubin AG, Berman ME, Narayanan R, Hundeyin M, Miller G
The Journal of experimental medicine 2017 Jun 5;214(6):1711-1724
The Journal of experimental medicine 2017 Jun 5;214(6):1711-1724
Intestinal fungi contribute to development of alcoholic liver disease.
Yang AM, Inamine T, Hochrath K, Chen P, Wang L, Llorente C, Bluemel S, Hartmann P, Xu J, Koyama Y, Kisseleva T, Torralba MG, Moncera K, Beeri K, Chen CS, Freese K, Hellerbrand C, Lee SM, Hoffman HM, Mehal WZ, Garcia-Tsao G, Mutlu EA, Keshavarzian A, Brown GD, Ho SB, Bataller R, Stärkel P, Fouts DE, Schnabl B
The Journal of clinical investigation 2017 Jun 30;127(7):2829-2841
The Journal of clinical investigation 2017 Jun 30;127(7):2829-2841
Knockout of Ccr2 alleviates photoreceptor cell death in rodent retina exposed to chronic blue light.
Hu Z, Zhang Y, Wang J, Mao P, Lv X, Yuan S, Huang Z, Ding Y, Xie P, Liu Q
Cell death & disease 2016 Nov 10;7(11):e2468
Cell death & disease 2016 Nov 10;7(11):e2468
Suppression of NLRP3 inflammasome by γ-tocotrienol ameliorates type 2 diabetes.
Kim Y, Wang W, Okla M, Kang I, Moreau R, Chung S
Journal of lipid research 2016 Jan;57(1):66-76
Journal of lipid research 2016 Jan;57(1):66-76
Chinese Herbal Preparation Xuebijing Potently Inhibits Inflammasome Activation in Hepatocytes and Ameliorates Mouse Liver Ischemia-Reperfusion Injury.
Liu X, Hu Z, Zhou B, Li X, Tao R
PloS one 2015;10(7):e0131436
PloS one 2015;10(7):e0131436
The cyclopentenone prostaglandin 15d-PGJ2 inhibits the NLRP1 and NLRP3 inflammasomes.
Maier NK, Leppla SH, Moayeri M
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 15;194(6):2776-85
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 15;194(6):2776-85
Caspase-8 as an Effector and Regulator of NLRP3 Inflammasome Signaling.
Antonopoulos C, Russo HM, El Sanadi C, Martin BN, Li X, Kaiser WJ, Mocarski ES, Dubyak GR
The Journal of biological chemistry 2015 Aug 14;290(33):20167-84
The Journal of biological chemistry 2015 Aug 14;290(33):20167-84
ASC in renal collecting duct epithelial cells contributes to inflammation and injury after unilateral ureteral obstruction.
Komada T, Usui F, Shirasuna K, Kawashima A, Kimura H, Karasawa T, Nishimura S, Sagara J, Noda T, Taniguchi S, Muto S, Nagata D, Kusano E, Takahashi M
The American journal of pathology 2014 May;184(5):1287-98
The American journal of pathology 2014 May;184(5):1287-98
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse NLRP3/NALP3 by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for NLRP3/NALP3 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Catalog # AF6789 is recommended for use with human lysates.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- NLRP3/NALP3 in RAW 264.7 Mouse Cell Line. NLRP3/NALP3 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of NLRP3/NALP3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse NLRP3/ NALP3 Monoclonal Antibody (Catalog # MAB7578, filled histo-gram) or isotype control antibody (Catalog # MAB006, open histo-gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of NLRP3/NALP3 in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes was stained with Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
