- PA5-35145 - Invitrogen Antibodies FOXD1 antibody

Cai K, Chen S, Zhu C, Li L, Yu C, He Z, Sun C
Cell death & disease 2022 Sep 3;13(9):765

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of FOXD1 in HepG2 cell lysate (35 µg/lane) using a FOXD1 polyclonal antibody (Product # PA5-35145).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of FOXD1 showing staining in the nucleus of B-3 cells using a FOXD1 polyclonal antibody (Product # PA5-35145) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/mL, 1 h at 37ºC).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of FOXD1 in formalin-fixed, paraffin-embedded human kidney tissue using a FOXD1 polyclonal antibody (Product # PA5-35145) followed by peroxidase conjugation of the secondary antibody and DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 FOXD1 is overexpressed in PC tissues and cells. A The intersection between differentially expressed genes (DEGs) in GSE16515 and FOX family members, represented as a Venn diagram. B Volcano map of the DEGs in GSE16515. C Overall survival curve and disease-free survival curve of FOXD1 differentially expressed PC patients based on TCGA database. D Relative expression of FOXD1 in 179 PC(T) tissues and 171 non-tumor (N) tissues based on TCGA and GTEx database. E GSEA analysis of FOXD1 expression-related genes. F Relative FOXD1 expression in 40 pairs of PC (T) tissues and non-tumor (N) tissues detected by RT-qPCR. G Relative FOXD1 expression in 90 pairs PC (T) tissues and non-tumor (N) tissues detected by immunohistochemistry (IHC). H Overall survival curve of 90 PC patients classified by IHC score. I Relative FOXD1 expression in HPNE cells and PC cells detect by RT-qPCR. J Relative FOXD1 expression in HPNE cells and PC cells detect by western blotting analysis. * p < 0.05; ** p < 0.01.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 3 FOXD1 promotes PC cell proliferation and metastasis in vivo. A Excised subcutaneous tumors 30 days after inoculation with the indicated PANC-1 cells. B Growth curves of subcutaneous tumor volume in each group. C Weight of subcutaneous tumors in each group. D Representative images of FOXD1, KI67, PCNA and GLUT1 expression in subcutaneous tumors detected through IHC. E Representative images of pulmonary metastases in each group. * p < 0.05; ** p < 0.01.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 7 FOXD1 expression correlates with GLUT1 in PC. A Representative image of FOXD1, HOXA11-AS, miR-148b-3p, and GLUT1 expression in serial sections of pancreatic cancer specimens detected through IHC and ISH. Pearson's chi-squared test of the expression of 40 specimens. B GLUT1 expression of MIA PaCa-2 and PANC-1 cells with FOXD1 overexpression and knock-down were detected using western blotting. C Illustration of how FOXD1 facilitates pancreatic cancer cell proliferation, invasion, and metastasis by regulating GLUT1-mediated aerobic glycolysis, created by Figdraw ( http://www.figdraw.com ). * p < 0.05; ** p < 0.01.

PA5-35145