HPA050786

antibody from Atlas Antibodies

Targeting: SPEF1 C20orf28, CLAMP, DKFZP434I114, SPEF1A

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA050786

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA050786, RRID:AB_2681238

Product name

Anti-SPEF1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

RDFSDGVLVAEVIKFYFPKMVEMHNYVPANSLQQK
LSNWGHLNRKVLKRLNFSVPDDVMRKIAQCAPGVV
ELVLIPLRQRLEERQRRRKQGAGSLQELAPQDGSG
YMDVGKVAFSISPSRL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human fallopian tube and colon tissues using Anti-SPEF1 antibody. Corresponding SPEF1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows low expression as expected.

Sample type

HUMAN