AM09004PU-S

antibody from OriGene

Targeting: CLU APOJ, CLI, CLU1, CLU2, KUB1, SGP-2, SP-40, TRPM-2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AM09004PU-S

Provider

OriGene

Product name

Clusterin (CLU) mouse monoclonal antibody, clone 1A11, Purified

Antibody type

Monoclonal

Description

Clusterin (CLU) mouse monoclonal antibody, clone 1A11, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

CLU

Isotype

IgG

Antibody clone number

1A11

Vial size

50 µl

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis: Cell lysates of U87MG(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Human Clusterin antibody -N (1/1000). Proteins were visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry: Paraffin embedded sections of human renal cell carcinoma tissue were incubated with anti-human Clusterin antibody -N (1/100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry: -N Clusterin antibody staining of Formalin-Fixed, Paraffin-Embedded Human Prostate at 10 ug/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry: -N Clusterin antibody staining of Formalin-Fixed, Paraffin-Embedded Human Lung at 10 ug/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

Validation comment

IHC