- PA5-29846 - Invitrogen Antibodies TPM1 antibody

Shen S, Sewanan LR, Shao S, Halder SS, Stankey P, Li X, Campbell SG
Stem cell reports 2022 Sep 13;17(9):2037-2049

Lin J, Shen J, Yue H, Cao Z
Oncology reports 2019 Dec;42(6):2371-2381

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Tropomyosin 1 using 30 µg of A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Tropomyosin 1 polyclonal antibody (Product # PA5-29846) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using TPM1 Polyclonal Antibody (Product # PA5-29846). Sample (50 µg of whole cell lysate). Lane A: mouse liver. 12% SDS PAGE. TPM1 Polyclonal Antibody (Product # PA5-29846) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using TPM1 Polyclonal Antibody (Product # PA5-29846). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TPM1 Polyclonal Antibody (Product # PA5-29846) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: TPM1 Polyclonal Antibody detects TPM1 protein by western blot analysis. A. 50 µg rat muscle lysate/extract.10% SDS-PAGE. TPM1 Polyclonal Antibody (Product # PA5-29846) dilution: 1:6,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of U-2 OS (Lane 1), A549 (Lane 2), K-562 (Lane 3), HL-60 (Lane 4), Raji (Lane 5), tissue extracts of Mouse Heart (Lane 6), Rat Heart (Lane 7), Mouse Skeletal Muscle (Lane 8) and Rat Skeletal Muscle (Lane 9). The blot was probed with Anti-TPM1 Polyclonal Antibody (Product # PA5-29846, 1:6000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 35 kDa band corresponding to TPM1 was observed in U-2 OS, A549 and tissue extracts tested except K-562, HL-60, Raji which are reported negative for TPM1 expression.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: TPM1 Polyclonal Antibody detects Tropomyosin 1 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Tropomyosin 1 stained by TPM1 Polyclonal Antibody (Product # PA5-29846) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of TPM1 was performed using 70% confluent log phase HSkMs cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TPM1 Rabbit Polyclonal Antibody (Product # PA5-29846) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Tropomyosin 1 (Product # PA5-29846) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: TPM1 Polyclonal Antibody detects Tropomyosin 1 protein at cytosol on mouse vein by immunohistochemical analysis. Sample: Paraffin-embedded mouse vein. TPM1 Polyclonal Antibody (Product # PA5-29846) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: TPM1 Polyclonal Antibody detects Tropomyosin 1 protein at cytosol on mouse vein by immunohistochemical analysis. Sample: Paraffin-embedded mouse vein. TPM1 Polyclonal Antibody (Product # PA5-29846) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1. Expression of miR-183-5p.1 and TPM1 in GC cells and tissues. miR-183-5p.1 levels in (A) normal tissues, adjacent tissues, GC tissues and (B) normal gastric epithelial cells (GES-1) and GC cell lines (MKN-7, AGS, HGC-27) were determined by RT-qPCR. TPM1 mRNA expression in (C) normal tissues, adjacent tissues, GC tissues and (D) GES-1, MKN-7, AGS, HGC-27 cells as quantified by RT-qPCR. (E) TPM1 protein expression in GES-1 and AGS cells was determined by western blotting and (H) statistically analyzed. (F) TPM1 protein expression in GES-1 and MKN-7 cells was determined by western blotting and (I) statistically analyzed. (G) TPM1 protein expression in GES-1 and HGC-27 cells was determined by western blotting and (J) statistically analyzed. Results are presented as the mean +- standard error of the mean (n=3) *P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3. Experimental validation of miR-183-5p.1 as a TPM1 regulator. (A) miR-183-5p.1 binding with the 3'UTR of TPM1 predicted by TargetScan and coding sequence of miR-183-5p.1 and TPM1. The luciferase activities of WT-TPM1 mRNAs in GC (B) AGS and (J) HGC-27 cells were significantly enhanced by transfection with miR-183-5p.1 inhibitors and markedly decreased in (F) AGS and (O) HGC-27 cells by miR-183-5p.1 mimics following the Luciferase reporter assay. TPM1 expression was markedly increased in (C-E) AGS and (K-N) HGC-27 cells by miR-183-5p.1 inhibitors and significantly decreased in (G-I) AGS and (P-R) HGC-27 cells by miR-183-5p.1 mimics as revealed by reverse transcription-quantitative PCR and western blotting. GAPDH served as the internal control. Results are presented as the mean +- standard error of the mean (n=3). *P

PA5-29846