Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-48187 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PATL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The Recurrent Mutation in PATL2 Inhibits Its Degradation Thus Causing Female Infertility Characterized by Oocyte Maturation Defect Through Regulation of the Mos-MAPK Pathway.
Cao Q, Zhao C, Wang C, Cai L, Xia M, Zhang X, Han J, Xu Y, Zhang J, Ling X, Ma X, Huo R
Frontiers in cell and developmental biology 2021;9:628649
Frontiers in cell and developmental biology 2021;9:628649
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PATL2 in 293 cell line lysates (35 µg/lane). Lysates were probed with a PATL2 Antibody (Center) (Product # PA5-48187).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PATL2 in 293 cells. Cells were stained with a PATL2 Antibody (Center) (Product # PA5-48187) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PATL2 in formalin fixed and paraffin embedded human spleen tissue. Samples were probed with a PATL2 Antibody (Center) (Product # PA5-48187) followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effects of Y217N variants on PATL2 degradation. (A,B) The Patl2 levels in oocytes at germinal vesicle (GV), meiosis metaphase I (MI), and meiosis metaphase II (MII) stages were determined by western blot. (C) Immunostaining of Patl2 in oocytes at GV, MI, and MII stages. Scale bar, 20 mum. (D) Ubiquitination was evaluated by co-IP with an anti-FLAG antibody. (E,F) The PATL2 stability comparison between group of PATL2 WT or PATL2 Y217N transfected cells is followed by CHX treatment. *** p < 0.001.