MA5-47160

antibody from Invitrogen Antibodies

Targeting: KDM6A UTX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-47160

Provider

Invitrogen Antibodies

Product name

KDM6A Monoclonal Antibody (HL1731)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Store as concentrated solution.

Antibody clone number

HL1731

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KDM6A in Various whole cell extracts (30 µg) separated by 5% SDS-PAGE. Membrane was blotted with KDM6A Monoclonal antibody (Product # MA5-47160) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KDM6A in Various whole cell extracts (30 µg) separated by 5% SDS-PAGE. Membrane was blotted with KDM6A Monoclonal antibody (Product # MA5-47160) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KDM6A in Various whole cell extracts (30 µg) separated by 5% SDS-PAGE. Membrane was blotted with KDM6A Monoclonal antibody (Product # MA5-47160) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KDM6A in MCF-7 cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KDM6A stained by KDM6A monoclonal antibody (Product # MA5-47160) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000. Blue: Fluoroshield with DAPI. Scale bar= 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KDM6A in paraffin-embedded human breast carcinoma. Samples were incubated in KDM6A Monoclonal antibody (Product # MA5-47160). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.