NBP1-80628

antibody from Novus Biologicals

Targeting: KDM6A UTX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-80628

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-80628, RRID:AB_11027990

Product name

Rabbit Polyclonal KDM6A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse KDM6A antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness.

Lu H, Xie Y, Tran L, Lan J, Yang Y, Murugan NL, Wang R, Wang YJ, Semenza GL
The Journal of clinical investigation 2020 Jul 27;

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Cancer-derived UTX TPR mutations G137V and D336G impair interaction with MLL3/4 complexes and affect UTX subcellular localization.

Kato H, Asamitsu K, Sun W, Kitajima S, Yoshizawa-Sugata N, Okamoto T, Masai H, Poellinger L
Oncogene 2020 Apr;39(16):3322-3335

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The H3K27me3-demethylase KDM6A is suppressed in breast cancer stem-like cells, and enables the resolution of bivalency during the mesenchymal-epithelial transition.

Taube JH, Sphyris N, Johnson KS, Reisenauer KN, Nesbit TA, Joseph R, Vijay GV, Sarkar TR, Bhangre NA, Song JJ, Chang JT, Lee MG, Soundararajan R, Mani SA
Oncotarget 2017 Sep 12;8(39):65548-65565

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KDM6A Antibody [NBP1-80628] - Analysis in human cell line HEK 293.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Red: UTX nuclear colocalization with DAPI (in blue). This image was submitted via customer Review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human bone marrow shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human pancreas shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human cervix shows moderate to strong nuclear positivity in squamous epithelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining in human bone marrow and skeletal muscle tissues using NBP1-80628 antibody. Corresponding KDM6A RNA-seq data are presented for the same tissues.