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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-31772 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KDM6A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using KDM6A Polyclonal Antibody (Product # PA5-31772). Sample (30 µg of whole cell lysate). Lane A: IMR32. Lane B: U87-MG. 5% SDS PAGE. KDM6A Polyclonal Antibody (Product # PA5-31772) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of KDM6A was achieved by transfecting MCF7 with KDM6A specific siRNAs (Silencer® select Product # s531230; s531232). Western blot analysis (Fig. a) was performed using whole cell extracts from the KDM6A knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with KDM6A Polyclonal Antibody (Product # PA5-31772, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to KDM6A.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of LNCaP (Lane 1), U-87 MG (Lane 2), HeLa (Lane 3), MCF7 (Lane 4) and SK-N-AS (Lane 5). The blot was probed with Anti-KDM6A Polyclonal Antibody (Product # PA5-31772, 1:1000 dilution) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 150kDa band corresponding to KDM6A was observed across all the cell lines tested. Some uncharacterized bands were observed between 50-60kDa.
