TA324613

antibody from OriGene

Targeting: HEYL bHLHb33, HESR3, HEY3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324613

Provider

OriGene

Product name

Rabbit polyclonal HEYL Antibody (N-term)

Antibody type

Polyclonal

Description

Rabbit polyclonal HEYL Antibody (N-term)

Host

Rabbit

Conjugate

Unconjugated

Epitope

HEYL

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

0.5 mg/ml

References

Submitted references

Novel potential predictive markers of sunitinib outcomes in long-term responders versus primary refractory patients with metastatic clear-cell renal cell carcinoma.

Puente J, Laínez N, Dueñas M, Méndez-Vidal MJ, Esteban E, Castellano D, Martinez-Fernández M, Basterretxea L, Juan-Fita MJ, Antón L, León L, Lambea J, Pérez-Valderrama B, Vázquez S, Suarez C, Del Muro XG, Gallardo E, Maroto JP, Samaniego ML, Suárez-Paniagua B, Sanz J, Paramio JM, SOGUG (Spanish Oncology Genitourinary Group)
Oncotarget 2017 May 2;8(18):30410-30421

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEYL Antibody (N-term) (Cat. #TA324613) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HEYL antibody detected the HEYL protein (arrow).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of Hela cell stained with HEYL Antibody (N-term)(Cat#TA324613).HeLa cells were incubated with HEYL primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . HEYL immunoreactivity is localized to Mitochondria significantly.

Validation comment

IF