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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA1-91639 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSPA9 Monoclonal Antibody (30A5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Western blot detects a band ~75 kDa. A suggested positive control is HeLa cell lysate (heat shocked).
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster, Porcine, Rabbit, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 30A5
- Vial size
- 25 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A Proteomic Study Suggests Stress Granules as New Potential Actors in Radiation-Induced Bystander Effects.
75-kDa glucose-regulated protein (GRP75) is a novel molecular signature for heat stress response in avian species.
Tudor M, Gilbert A, Lepleux C, Temelie M, Hem S, Armengaud J, Brotin E, Haghdoost S, Savu D, Chevalier F
International journal of molecular sciences 2021 Jul 26;22(15)
International journal of molecular sciences 2021 Jul 26;22(15)
75-kDa glucose-regulated protein (GRP75) is a novel molecular signature for heat stress response in avian species.
Dhamad AE, Greene E, Sales M, Nguyen P, Beer L, Liyanage R, Dridi S
American journal of physiology. Cell physiology 2020 Feb 1;318(2):C289-C303
American journal of physiology. Cell physiology 2020 Feb 1;318(2):C289-C303
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of HSPA9 was achieved by transfecting HeLa cells with HSPA9 specific siRNAs (Silencer® select Product # s6990, s6988). Western blot analysis (Fig. a) was performed using whole cell extracts from the HSPA9 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with HSPA9 Monoclonal Antibody (Product # MA1-91639, 1:3000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HSPA9.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSPA9 in various cells using HSPA9 Monoclonal Antibody (30A5) (Product # MA1-91639). Lane 1: MW marker, Lane 2: HeLa (heat shocked), Lane 3: L-929 (heat shocked), Lane 4: CHO -K1 (heat shocked), Lane 5: GPC-16 (heat shocked), Lane 6: PC-12 (heat shocked).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HepG2 (Lane 2), NIH3T3 (Lane 3), tissue extracts of Mouse Liver (Lane 4), Rat Liver (Lane 5), Mouse Brain (Lane 6) and Rat Brain (Lane 7). The blot was probed with Anti-HSPA9 Monoclonal Antibody (Product # MA1-91639, 1:3000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 70 kDa band corresponding to HSPA9 was observed across all the cell lines and tissues tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HSPA9 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSPA9 Monoclonal Antibody (30A5) (Product # MA1-91639) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using HSPA9 Monoclonal Antibody (30A5) (Product # MA1-91639).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Western blotting analysis of cyclophilin A, thioredoxin, alpha-enolase, RPLP0, HSC70, HSPA9, and CCT3 in a whole-cell extracts from T/C-28A2 bystander cells receiving the conditioned medium of low-dose irradiated chondrosarcoma cells (0.1 Gy) or non-irradiated chondrosarcoma cells (CTR).
