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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- GTX104407 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104407, RRID:AB_1950527
- Product name
- Grp75 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
Submitted references Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer.
Effect of glutathione reductase knockdown in response to UVB-induced oxidative stress in human lung adenocarcinoma.
Lo YW, Lin ST, Chang SJ, Chan CH, Lyu KW, Chang JF, May EW, Lin DY, Chou HC, Chan HL
Journal of cellular and molecular medicine 2015 Apr;19(4):744-59
Journal of cellular and molecular medicine 2015 Apr;19(4):744-59
Effect of glutathione reductase knockdown in response to UVB-induced oxidative stress in human lung adenocarcinoma.
Chou HC, Chan HL
Proteome science 2014 Jan 9;12(1):2
Proteome science 2014 Jan 9;12(1):2
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX104407 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ug whole cell lysate)A: 293TB: Raji (GTX27908)7.5% SDS PAGEGTX104407 diluted at 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GRP75 antibody detects GRP75 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody (GTX104407) diluted at a dilution of 1:2500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GRP75 antibody detects GRP75 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody (GTX104407) diluted at a dilution of 1:500.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (6 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody (GTX104407) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody (GTX104407) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Grp75 antibody detects Grp75 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Grp75 stained by Grp75 antibody (GTX104407) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of GRP75 protein from HepG2 whole cell extracts using 5 £gg of GRP75 antibody (GTX104407).Western blot analysis was performed using GRP75 antibody (GTX104407).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Grp75 antibody detects Grp75 protein at mitochondria in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Grp75 antibody (GTX104407) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Grp75 antibody detects Grp75 protein at mitochondria in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. Grp75 antibody (GTX104407) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Grp75 antibody detects Grp75 protein at mitochondria in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Grp75 antibody (GTX104407) diluted at 1:500.
