PAB29350

antibody from Abnova Corporation

Targeting: HSPA9 GRP75, HSPA9B, mot-2, mthsp75, PBP74

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29350

Provider

Abnova Corporation

Product name

HSPA9 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human HSPA9.

Antigen sequence

GIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMV
KNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKME
EFKDQLPADECNKLKEEISKMRELLARKDSETGEN
IRQAASSLQQASLKLFEMAYKKMASEREGSGSSGT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with HSPA9 polyclonal antibody (Cat # PAB29350) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with HSPA9 polyclonal antibody (Cat # PAB29350) at 1-4 ug/mL concentration shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with HSPA9 polyclonal antibody (Cat # PAB29350) shows strong cytoplasmic positivity in neuronal cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)