Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP1-21670 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-21670, RRID:AB_1660156
- Product name
- Mouse Monoclonal GRP75/HSPA9B/Mortalin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 75kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: GRP75/HSPA9B/Mortalin Antibody (S52A-42) [NBP1-21670] - analysis of Human HeLa cell lysates showing detection of Grp75 protein using Mouse Anti-Grp75 Monoclonal Antibody, Clone S52A-42 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Grp75 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.