Explore
Validate
Learn
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB2339 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2339, RRID:AB_1575873
- Product name
- HDAC9 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of HDAC9.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular characterization of a familial translocation implicates disruption of HDAC9 and possible position effect on TGFbeta2 in the pathogenesis of Peters' anomaly.
The histone deacetylase 9 gene encodes multiple protein isoforms.
Chromosomal organization and localization of the human histone deacetylase 9 gene (HDAC9).
David D, Cardoso J, Marques B, Marques R, Silva ED, Santos H, Boavida MG
Genomics 2003 May;81(5):489-503
Genomics 2003 May;81(5):489-503
The histone deacetylase 9 gene encodes multiple protein isoforms.
Petrie K, Guidez F, Howell L, Healy L, Waxman S, Greaves M, Zelent A
The Journal of biological chemistry 2003 May 2;278(18):16059-72
The Journal of biological chemistry 2003 May 2;278(18):16059-72
Chromosomal organization and localization of the human histone deacetylase 9 gene (HDAC9).
Mahlknecht U, Schnittger S, Will J, Cicek N, Hoelzer D
Biochemical and biophysical research communications 2002 Apr 26;293(1):182-91
Biochemical and biophysical research communications 2002 Apr 26;293(1):182-91
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Both anti-HDAC9 N-term (Cat # PAB2238) and C-term (Cat # PAB2339) polyclonal antibody were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells.Top figure shows both polyclonal antibody specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.Bottom figure shows that both polyclonal antibody can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL) .
- Validation comment
- Immunoprecipitation
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the HDAC9 polyclonal antibody (Cat # PAB2339) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
