AF2805
antibody from R&D Systems
Targeting: MFGE8
BA46, EDIL1, hP47, HsT19888, MFG-E8, OAcGD3S, SED1, SPAG10
Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF2805 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse MFG-E8 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse MFG-E8 in direct ELISAs and Western blots. In these formats, less than 2% cross-reactivity with recombinant human MFG-E8 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P21956
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Anti-HER2 scFv-Directed Extracellular Vesicle-Mediated mRNA-Based Gene Delivery Inhibits Growth of HER2-Positive Human Breast Tumor Xenografts by Prodrug Activation.
Obesity and Insulin Resistance Promote Atherosclerosis through an IFNγ-Regulated Macrophage Protein Network.
Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia.
Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release.
Developmental Emergence of Adult Neural Stem Cells as Revealed by Single-Cell Transcriptional Profiling.
Spherical nucleic acid targeting microRNA-99b enhances intestinal MFG-E8 gene expression and restores enterocyte migration in lipopolysaccharide-induced septic mice.
MFG-E8 regulates microglial phagocytosis of apoptotic neurons.
Wang JH, Forterre AV, Zhao J, Frimannsson DO, Delcayre A, Antes TJ, Efron B, Jeffrey SS, Pegram MD, Matin AC
Molecular cancer therapeutics 2018 May;17(5):1133-1142
Molecular cancer therapeutics 2018 May;17(5):1133-1142
Obesity and Insulin Resistance Promote Atherosclerosis through an IFNγ-Regulated Macrophage Protein Network.
Reardon CA, Lingaraju A, Schoenfelt KQ, Zhou G, Cui C, Jacobs-El H, Babenko I, Hoofnagle A, Czyz D, Shuman H, Vaisar T, Becker L
Cell reports 2018 Jun 5;23(10):3021-3030
Cell reports 2018 Jun 5;23(10):3021-3030
Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia.
Brelstaff J, Tolkovsky AM, Ghetti B, Goedert M, Spillantini MG
Cell reports 2018 Aug 21;24(8):1939-1948.e4
Cell reports 2018 Aug 21;24(8):1939-1948.e4
Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release.
Obata Y, Kita S, Koyama Y, Fukuda S, Takeda H, Takahashi M, Fujishima Y, Nagao H, Masuda S, Tanaka Y, Nakamura Y, Nishizawa H, Funahashi T, Ranscht B, Izumi Y, Bamba T, Fukusaki E, Hanayama R, Shimada S, Maeda N, Shimomura I
JCI insight 2018 Apr 19;3(8)
JCI insight 2018 Apr 19;3(8)
Developmental Emergence of Adult Neural Stem Cells as Revealed by Single-Cell Transcriptional Profiling.
Yuzwa SA, Borrett MJ, Innes BT, Voronova A, Ketela T, Kaplan DR, Bader GD, Miller FD
Cell reports 2017 Dec 26;21(13):3970-3986
Cell reports 2017 Dec 26;21(13):3970-3986
Spherical nucleic acid targeting microRNA-99b enhances intestinal MFG-E8 gene expression and restores enterocyte migration in lipopolysaccharide-induced septic mice.
Wang X, Hao L, Bu HF, Scott AW, Tian K, Liu F, De Plaen IG, Liu Y, Mirkin CA, Tan XD
Scientific reports 2016 Aug 19;6:31687
Scientific reports 2016 Aug 19;6:31687
MFG-E8 regulates microglial phagocytosis of apoptotic neurons.
Fuller AD, Van Eldik LJ
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2008 Dec;3(4):246-56
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2008 Dec;3(4):246-56
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse MFG-E8 by Western Blot. Western blot shows lysates of mouse mammary gland tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MFG-E8 at approximately 60-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse MFG-E8 by Simple WesternTM. Simple Western lane view shows lysates of recombinant mouse (rm) MFG-E8, loaded at 50 ng/mL. A specific band was detected for MFG-E8 at approximately 87 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.