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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA302489 - Provider product page
- Provider
- OriGene
- Product name
- Goat Polyclonal Antibody against Flotillin 1
- Antibody type
- Polyclonal
- Description
- Goat Polyclonal Antibody against Flotillin 1
- Host
- Goat
- Conjugate
- Unconjugated
- Epitope
- FLOT1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TA302489 staining (0.3ug/ml) of H460 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293 overexpressing FLOT1(RC200231) and probed with TA302489 (mock transfection in first lane).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TA302489 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- IHC
