849801

antibody from BioLegend

Targeting: FLOT1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

849801

Provider

BioLegend

Proper citation

BioLegend Cat#849801, RRID:AB_2687308

Product name

Purified anti-Flotillin-1

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

W16108A

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Western blot of anti-Flotillin-1 antibody (clone W16108A) and isotype-matched IgG2a control. Blots were incubated with 5µg/ml (left) or 0.5µg/ml (middle) anti-Flotillin-1 antibody, or rat IgG2a, κ isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system. M: molecular weight marker; brain lysates: 20µg; Myc-tagged Flotillin-1 or GST-tagged Flotillin-2 recombinant protein: 20 ng.

Conjugate

Unconjugated