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Learn15571-1-AP
antibody from Invitrogen Antibodies
Targeting: FLOT1
Western blot Immunocytochemistry
Immunoprecipitation
Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 15571-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Flotillin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLAAACQMF LGKTEAEIAH IALETLEGHQ RAIMAHMTVE EIYKDRQKFS EQVFKVASSD LVNMGISVVS YTLKDIHDDQ DYLHSLGKAR TAQVQKDARI GEAEAKRDAG IREAKAKQEK VSAQYLSEIE MAKAQRDYEL KKAAYDIEVN TRRAQADLAY QLQVAKTKQQ IEEQRVQVQV VERAQQVAVQ EQEIARREKE LEARVRKPAE AERYKLERLA EAEKSQLIMQ AEAEAASVRM RGEAEAFAIG ARARAEAEQM AKKAEAFQLY QEAAQLDMLL EKLPQVAEEI SGPLTSANKI TLVSSGSGTM GAAKVTGEVL DILTRLPESV ERLTGVSISQ VNHKPLRTA (80-427 aa encoded by BC001146)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 15571-1-AP (Flotillin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 15571-1-AP (Flotillin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using FLOT1 antibody 15571-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.05ug Flotillin 1 antibody (15571-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488 -Goat anti-Rabbit IGG with dilution 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Flotillin 1 (IP:15571-1-AP, 4ug; Detection:15571-1-AP 1:500) with mouse brain tissue lysate 4400ug.
