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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP16027PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP16027PU-N, RRID:AB_1927997
- Product name
- anti Flotillin-1 / FLOT1 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence C-SISQVNHKPLRTA, from the C-Terminus of the protein sequence according to NP_005794.1.
- Reactivity
- Human, Bovine, Canine, Porcine
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
Submitted references Lipid rafts mediate internalization of beta1-integrin in migrating intestinal epithelial cells.
Vassilieva EV, Gerner-Smidt K, Ivanov AI, Nusrat A
American journal of physiology. Gastrointestinal and liver physiology 2008 Nov;295(5):G965-76
American journal of physiology. Gastrointestinal and liver physiology 2008 Nov;295(5):G965-76
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16027PU-N staining (0.3µg/ml) of H460 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HEK293 overexpressing FLOT1 and probed with AP16027PU-N (mock transfection in first lane), tested by Origene.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16027PU-N staining (3.8 µg/ml) of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
