Antibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AF2519 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD36/SR-B3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CD36/SR-B3 in ELISAs and Western blots. In sandwich ELISAs, approximately 20% cross-reactivity with recombinant human CD36 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q3UAI3
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A role for scavenger receptor B1 as a captor of specific fatty acids in taste buds of circumvallate papillae.
A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity.
Metabolomic insights of macrophage responses to graphene nanoplatelets: Role of scavenger receptor CD36.
IL-34 promotes foam cell formation by enhancing CD36 expression through p38 MAPK pathway.
Vimentin deficiency in macrophages induces increased oxidative stress and vascular inflammation but attenuates atherosclerosis in mice.
Partitioning of adipose lipid metabolism by altered expression and function of PPAR isoforms after bariatric surgery.
Inhibition of the CD36 receptor reduces visceral fat accumulation and improves insulin resistance in obese mice carrying the BDNF-Val66Met variant.
CD36 is essential for endurance improvement, changes in whole-body metabolism, and efficient PPAR-related transcriptional responses in the muscle with exercise training.
Intestinal epithelial cell caveolin 1 regulates fatty acid and lipoprotein cholesterol plasma levels.
CCN3 Regulates Macrophage Foam Cell Formation and Atherosclerosis.
CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide.
Cell Surface CD36 Protein in Monocyte/Macrophage Contributes to Phagocytosis during the Resolution Phase of Ischemic Stroke in Mice.
Expression of CD36 by Olfactory Receptor Cells and Its Abundance on the Epithelial Surface in Mice.
Regulation of AMPK activation by CD36 links fatty acid uptake to β-oxidation.
CD36 protein influences myocardial Ca2+ homeostasis and phospholipid metabolism: conduction anomalies in CD36-deficient mice during fasting.
Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice.
Deficiency in the nuclear factor E2-related factor-2 transcription factor results in impaired adipogenesis and protects against diet-induced obesity.
Silencing of either SR-A or CD36 reduces atherosclerosis in hyperlipidaemic mice and reveals reciprocal upregulation of these receptors.
Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages.
Transcriptional regulation of fatty acid translocase/CD36 expression by CCAAT/enhancer-binding protein alpha.
CD36/fatty acid translocase, an inflammatory mediator, is involved in hyperlipidemia-induced exacerbation in ischemic brain injury.
Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
Tsuzuki S, Lee S, Kimoto Y, Sugawara T, Manabe Y, Inoue K
Biomedical research (Tokyo, Japan) 2018;39(6):295-300
Biomedical research (Tokyo, Japan) 2018;39(6):295-300
A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity.
Tsuzuki S, Kimoto Y, Lee S, Sugawara T, Manabe Y, Inoue K
Biomedical research (Tokyo, Japan) 2018;39(3):117-129
Biomedical research (Tokyo, Japan) 2018;39(3):117-129
Metabolomic insights of macrophage responses to graphene nanoplatelets: Role of scavenger receptor CD36.
Adamson SX, Wang R, Wu W, Cooper B, Shannahan J
PloS one 2018;13(11):e0207042
PloS one 2018;13(11):e0207042
IL-34 promotes foam cell formation by enhancing CD36 expression through p38 MAPK pathway.
Liu Q, Fan J, Bai J, Peng L, Zhang T, Deng L, Wang G, Zhao Y, Nong J, Zhang M, Wang Y
Scientific reports 2018 Nov 26;8(1):17347
Scientific reports 2018 Nov 26;8(1):17347
Vimentin deficiency in macrophages induces increased oxidative stress and vascular inflammation but attenuates atherosclerosis in mice.
Håversen L, Sundelin JP, Mardinoglu A, Rutberg M, Ståhlman M, Wilhelmsson U, Hultén LM, Pekny M, Fogelstrand P, Bentzon JF, Levin M, Borén J
Scientific reports 2018 Nov 19;8(1):16973
Scientific reports 2018 Nov 19;8(1):16973
Partitioning of adipose lipid metabolism by altered expression and function of PPAR isoforms after bariatric surgery.
Jahansouz C, Xu H, Hertzel AV, Kizy S, Steen KA, Foncea R, Serrot FJ, Kvalheim N, Luthra G, Ewing K, Leslie DB, Ikramuddin S, Bernlohr DA
International journal of obesity (2005) 2018 Feb;42(2):139-146
International journal of obesity (2005) 2018 Feb;42(2):139-146
Inhibition of the CD36 receptor reduces visceral fat accumulation and improves insulin resistance in obese mice carrying the BDNF-Val66Met variant.
Yang J, Park KW, Cho S
The Journal of biological chemistry 2018 Aug 24;293(34):13338-13348
The Journal of biological chemistry 2018 Aug 24;293(34):13338-13348
CD36 is essential for endurance improvement, changes in whole-body metabolism, and efficient PPAR-related transcriptional responses in the muscle with exercise training.
Manio MCC, Matsumura S, Masuda D, Inoue K
Physiological reports 2017 May;5(10):e13282
Physiological reports 2017 May;5(10):e13282
Intestinal epithelial cell caveolin 1 regulates fatty acid and lipoprotein cholesterol plasma levels.
Otis JP, Shen MC, Quinlivan V, Anderson JL, Farber SA
Disease models & mechanisms 2017 Mar 1;10(3):283-295
Disease models & mechanisms 2017 Mar 1;10(3):283-295
CCN3 Regulates Macrophage Foam Cell Formation and Atherosclerosis.
Shi H, Zhang C, Pasupuleti V, Hu X, Prosdocimo DA, Wu W, Qing Y, Wu S, Mohammad H, Gerson SL, Perbal B, Klenotic PA, Dong N, Lin Z
The American journal of pathology 2017 Jun;187(6):1230-1237
The American journal of pathology 2017 Jun;187(6):1230-1237
CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide.
Biedroń R, Peruń A, Józefowski S
PloS one 2016;11(4):e0153558
PloS one 2016;11(4):e0153558
Cell Surface CD36 Protein in Monocyte/Macrophage Contributes to Phagocytosis during the Resolution Phase of Ischemic Stroke in Mice.
Woo MS, Yang J, Beltran C, Cho S
The Journal of biological chemistry 2016 Nov 4;291(45):23654-23661
The Journal of biological chemistry 2016 Nov 4;291(45):23654-23661
Expression of CD36 by Olfactory Receptor Cells and Its Abundance on the Epithelial Surface in Mice.
Lee S, Eguchi A, Tsuzuki S, Matsumura S, Inoue K, Iwanaga T, Masuda D, Yamashita S, Fushiki T
PloS one 2015;10(7):e0133412
PloS one 2015;10(7):e0133412
Regulation of AMPK activation by CD36 links fatty acid uptake to β-oxidation.
Samovski D, Sun J, Pietka T, Gross RW, Eckel RH, Su X, Stahl PD, Abumrad NA
Diabetes 2015 Feb;64(2):353-9
Diabetes 2015 Feb;64(2):353-9
CD36 protein influences myocardial Ca2+ homeostasis and phospholipid metabolism: conduction anomalies in CD36-deficient mice during fasting.
Pietka TA, Sulkin MS, Kuda O, Wang W, Zhou D, Yamada KA, Yang K, Su X, Gross RW, Nerbonne JM, Efimov IR, Abumrad NA
The Journal of biological chemistry 2012 Nov 9;287(46):38901-12
The Journal of biological chemistry 2012 Nov 9;287(46):38901-12
Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice.
Nagashima M, Watanabe T, Terasaki M, Tomoyasu M, Nohtomi K, Kim-Kaneyama J, Miyazaki A, Hirano T
Diabetologia 2011 Oct;54(10):2649-59
Diabetologia 2011 Oct;54(10):2649-59
Deficiency in the nuclear factor E2-related factor-2 transcription factor results in impaired adipogenesis and protects against diet-induced obesity.
Pi J, Leung L, Xue P, Wang W, Hou Y, Liu D, Yehuda-Shnaidman E, Lee C, Lau J, Kurtz TW, Chan JY
The Journal of biological chemistry 2010 Mar 19;285(12):9292-300
The Journal of biological chemistry 2010 Mar 19;285(12):9292-300
Silencing of either SR-A or CD36 reduces atherosclerosis in hyperlipidaemic mice and reveals reciprocal upregulation of these receptors.
Mäkinen PI, Lappalainen JP, Heinonen SE, Leppänen P, Lähteenvuo MT, Aarnio JV, Heikkilä J, Turunen MP, Ylä-Herttuala S
Cardiovascular research 2010 Dec 1;88(3):530-8
Cardiovascular research 2010 Dec 1;88(3):530-8
Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages.
Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y
Atherosclerosis 2009 Jun;204(2):405-11
Atherosclerosis 2009 Jun;204(2):405-11
Transcriptional regulation of fatty acid translocase/CD36 expression by CCAAT/enhancer-binding protein alpha.
Qiao L, Zou C, Shao P, Schaack J, Johnson PF, Shao J
The Journal of biological chemistry 2008 Apr 4;283(14):8788-95
The Journal of biological chemistry 2008 Apr 4;283(14):8788-95
CD36/fatty acid translocase, an inflammatory mediator, is involved in hyperlipidemia-induced exacerbation in ischemic brain injury.
Kim E, Tolhurst AT, Qin LY, Chen XY, Febbraio M, Cho S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 30;28(18):4661-70
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 30;28(18):4661-70
Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
Chang Y, Finnemann SC
Journal of cell science 2007 Sep 1;120(Pt 17):3053-63
Journal of cell science 2007 Sep 1;120(Pt 17):3053-63
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD36/SR-B3 in Mouse Kidney. CD36/SR-B3 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD36/SR-B3 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).