MAB6908

antibody from Abnova Corporation

Targeting: CD36 FAT, GP3B, GP4, GPIV, SCARB3

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6908

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB6908, RRID:AB_10678651

Product name

CD36 monoclonal antibody, clone SMO

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against native CD36.

Isotype

IgM

Antibody clone number

SMO

Storage

Store at 4°C. Do not freeze.

References

Submitted references

CD36 gene transfer confers capacity for phagocytosis of cells undergoing apoptosis.

Ren Y, Silverstein RL, Allen J, Savill J
The Journal of experimental medicine 1995 May 1;181(5):1857-62

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CD36 directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes.

Oquendo P, Hundt E, Lawler J, Seed B
Cell 1989 Jul 14;58(1):95-101

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Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.

Hogg N, MacDonald S, Slusarenko M, Beverley PC
Immunology 1984 Dec;53(4):753-67

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

5 X 10⁵ ficoll prepared human peripheral blood lymphocytes were washed and preincubated 5 minutes with 20 m l of 250 mg/mL human IgG (To block non specific binding) after which they were incubated 45 minutes on ice with 80m l of CD36 monoclonal antibody, clone SMO (Cat # MAB6908) at a concentration of 5 mg/mL. Cells were washed twice and incubated with Goat anti-Mouse Ig/FITC, after which they were washed three times, fixed and analyzed using a BD FACstar plus. A net 16% sub population of the cells stained positive with a mean shift of 1.05 log10 fluorescent units when compared to a Mouse IgM negative control at a similar concentration.

Validation comment

Flow Cytometry