- PA1-16813 - Invitrogen Antibodies CD36 antibody

Submitted references Oxidation of Hemoglobin Drives a Proatherogenic Polarization of Macrophages in Human Atherosclerosis.

Potor L, Hendrik Z, Patsalos A, Katona É, Méhes G, Póliska S, Csősz É, Kalló G, Komáromi I, Combi Z, Posta N, Sikura KÉ, Pethő D, Oros M, Vereb G, Tóth C, Gergely P, Nagy L, Balla G, Balla J
Antioxidants & redox signaling 2021 Oct 20;35(12):917-950

CTRP12 ameliorates atherosclerosis by promoting cholesterol efflux and inhibiting inflammatory response via the miR-155-5p/LXRα pathway.

Wang G, Chen JJ, Deng WY, Ren K, Yin SH, Yu XH
Cell death & disease 2021 Mar 10;12(3):254

Physiological Doses of Hydroxytyrosol Modulate Gene Expression in Skeletal Muscle of Exercised Rats.

Casuso RA, Al Fazazi S, Plaza-Díaz J, Ruiz-Ojeda FJ, Rueda-Robles A, Aragón-Vela J, Huertas JR
Life (Basel, Switzerland) 2021 Dec 12;11(12)

Sea-Buckthorn Seed Oil Induces Proliferation of both Normal and Dysplastic Keratinocytes in Basal Conditions and under UVA Irradiation.

Dudau M, Vilceanu AC, Codrici E, Mihai S, Popescu ID, Albulescu L, Tarcomnicu I, Moise G, Ceafalan LC, Hinescu ME, Enciu AM, Tanase C
Journal of personalized medicine 2021 Apr 7;11(4)

Prohibitin 2 deficiency impairs cardiac fatty acid oxidation and causes heart failure.

Wu D, Jian C, Peng Q, Hou T, Wu K, Shang B, Zhao M, Wang Y, Zheng W, Ma Q, Li CY, Cheng H, Wang X, Zhao L
Cell death & disease 2020 Mar 12;11(3):181

Hepatocyte-Specific Expression of Human Carboxylesterase 1 Attenuates Diet-Induced Steatohepatitis and Hyperlipidemia in Mice.

Xu Y, Zhu Y, Bawa FC, Hu S, Pan X, Yin L, Zhang Y
Hepatology communications 2020 Apr;4(4):527-539

Fatty acid transporter expression and regulation is impaired in placental macrovascular endothelial cells in obese women.

Yang X, Glazebrook P, Ranasinghe GC, Haghiac M, Calabuig-Navarro V, Minium J, O'Tierney-Ginn P
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 2019 Mar;32(6):971-978

Dysregulated Lipid Transport Proteins Correlate With Pathogenesis and Outcome in Severe Alcoholic Hepatitis.

Maras JS, Das S, Bhat A, Kumar Vyas A, Yadav G, Chaudhary S, Sukriti S, Gupta AC, Bihari C, Mahiwall R, Sarin SK
Hepatology communications 2019 Dec;3(12):1598-1625

Hepatic Forkhead Box Protein A3 Regulates ApoA-I (Apolipoprotein A-I) Expression, Cholesterol Efflux, and Atherogenesis.

Li Y, Xu Y, Jadhav K, Zhu Y, Yin L, Zhang Y
Arteriosclerosis, thrombosis, and vascular biology 2019 Aug;39(8):1574-1587

High-Content Screening of a Taiwanese Indigenous Plant Extract Library Identifies Syzygium simile leaf Extract as an Inhibitor of Fatty Acid Uptake.

Yen CH, Chang HS, Yang TH, Wang SF, Wu HC, Chen YC, Lin KJ, Wang S
International journal of molecular sciences 2018 Jul 22;19(7)

The intestinal microbiota regulates body composition through NFIL3 and the circadian clock.

Wang Y, Kuang Z, Yu X, Ruhn KA, Kubo M, Hooper LV
Science (New York, N.Y.) 2017 Sep 1;357(6354):912-916

Influence of high fat diet and resveratrol supplementation on placental fatty acid uptake in the Japanese macaque.

O'Tierney-Ginn P, Roberts V, Gillingham M, Walker J, Glazebrook PA, Thornburg KL, Grove K, Frias AE
Placenta 2015 Aug;36(8):903-10

Heterogeneity and immunophenotypic plasticity of malignant cells in human liposarcomas.

Zhang Y, Young ED, Bill K, Belousov R, Peng T, Lazar AJ, Pollock RE, Simmons PJ, Lev D, Kolonin MG
Stem cell research 2013 Sep;11(2):772-81

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western detection of CD36 in human adipocyte extract (30 µg). Lane 1: 0.5 µg/mL (Product # PA1-16813); lane 2: 2 µg/mL (Product # PA1-16813). ECL: 3 second exposure.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of CD36 in human Skin and Adipose tissue, mouse Adipose and Rat Adipose tissue. Samples were incubated in CD36 polyclonal antibody (Product # PA1-16813) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody. Separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CD36 in 30 µg human adipocyte. Samples were incubated in CD36 polyclonal antibody (Product # PA1-16813). Lane 1: 0.5 µg/mL primary antibody; Lane 2: 2 µg/mL primary antibody. ECL: 3 second exposure.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot was performed using Anti-CD36 Polyclonal Antibody (Product # PA1-16813) and a 88 kDa band corresponding to glycosylated form of Platelet glycoprotein 4 was observed in 3T3-L1 upon differentiation to adipocytes. Also, the protein expression was observed to be higher in adipose and brown adipose tissues when compared to other tissues tested. Membrane enriched extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2), Mouse Adipose (Lane 3), Mouse Brown Adipose (Lane 4), Rat Adipose (Lane 5), Rat Brown Adipose (Lane 6), Mouse Brain (Lane 7), Rat Brain (Lane 8), Mouse Kidney (Lane 9), Mouse Liver (Lane 10) and Rat Testis (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Detection of CD36 (Green) in HepG2 cells using Product # PA1-16813. Nuclei (Blue) were counterstained using Hoechst 33258.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of CD36 in human adipocytes. Samples were incubated in CD36 polyclonal antibody (Product # PA1-16813).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4 SSLE reduced the mRNA and protein expression of CD36 . ( A - E ) Huh7 cells were treated with 125 uM OA in the presence or absence of 100 ug/mL SSLE for 12 h. RT-qPCR was used to determine the expression profile of lipid metabolism genes. ( A ) Lipid entry: CD36 ; ( B ) TG formation: DGAT1 ; ( C ) beta-oxidation: CPT1 ; ( D ) lipid secretion: APOB and MTTP ; ( E ) mitochondria biogenesis: SIRT1 and PGC1 a . * significant difference from the DMSO control group (* p < 0.05, ** p < 0.01, *** p < 0.001, t -test); ( F ) Huh7 cells were treated as indicated for 12 h. Protein expression of CD36 was determined by immunoblot.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 2 Effects of CTRP12 on the expression of cholesterol efflux and uptake markers. A - D THP-1 macrophages were incubated with 50 ug/mL ox-LDL for 48 h, followed by treatment with PBS, LV-NC, or LV-CTRP12 for 72 h. A , B The expression of ABCA1 and ABCG1 was determined by qRT-PCR and western blot. C , D Detection of CD36 and SR-A expression using qRT-PCR and western blot. Data are expressed as the mean +- SD from three independent experiments. **** P < 0.0001; ns not significant.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 CD36 expression in normal (HEKa) and dysplastic (DOK) keratinocytes after UVA irradiation. Expression of CD36 in both HEKa and DOK cells was assessed by confocal immunofluorescence and confirmed by Western blot. In basal conditions, CD36 expression in normal keratinocytes is undetectable with the selected methods. Its expression increases in dysplastic cells, as well as following UVA irradiation.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 Quantitative proteomic analysis identifies significantly down-regulated lipid transporter proteins in SAH. (A) Partial list of proteins differentially regulated (>1.5-fold; P < 0.05) between SAH, AC, HC, and its GO analysis, which documents enrichment of lipid transporters in SAH. (B) KEGG enrichment analysis of the up-regulated (n = 32) and down-regulated (n = 176) proteins in patients with SAH represents significant ( P < 0.05) enrichment. (C) Plasma level of PON1 was down-regulated in patients with SAH (median, 23.2; range, 2.3-139.4) compared to those with AC (median, 44.7; range, 25.8-131) and HC (median, 139.5; range, 46.6-196.0) ( P < 0.05). (D) Plasma level of apolipoproteins (APOB, APOE, APOA1, APOA2, APOC1, APOC3) in SAH compared to AC and HC ( P < 0.05 is significant). (E) IHC showing expression of PON1, LDL, CD36, SRA1, ABCA1, and ABCG1 in SAH (n = 5) and AC (n = 5). For all IHC analyses, relative quantization of positively stained cells are expressed as mean number of positive cells/10 high-power field (40x) and * P < 0.05. Abbreviations: COA, coenzyme A; HBG1, hemoglobin subunit gamma 1; KEGG, Kyoto Encyclopedia of Genes and Genomes; LBP, lipopolysaccharide binding protein; LPS, lipopolysaccharide; TLR4, toll-like receptor 4;

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1. Localization of fatty acid transporters in human term placenta. Immunohistochemical analysis showed fatty acid translocase (FAT/CD36), plasma membrane associated fatty acid binding protein (FABPpm), fatty acid transport protein 4 (FATP4), and fatty acid binding protein (FABP4) localized in the syncytiotrophoblast layer (large arrows) and endothelial layer of the placental vasculature (small arrows). Scale bars = 10 um.

PA1-16813

Antibody data