PA5-29589

antibody from Invitrogen Antibodies

Targeting: CD36 FAT, GP3B, GP4, GPIV, SCARB3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29589

Provider

Invitrogen Antibodies

Product name

CD36 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, HepG2, Mouse brown adipose.

Concentration

0.71 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD36 Polyclonal Antibody (Product # PA5-29589). Sample (50 µg of whole cell lysate). Lane A: Mouse brown adipose . 7.5% SDS PAGE. CD36 Polyclonal Antibody (Product # PA5-29589) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD36 Polyclonal Antibody (Product # PA5-29589). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HepG2 . 7.5% SDS PAGE. CD36 Polyclonal Antibody (Product # PA5-29589) diluted at 1:2,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD36 Polyclonal Antibody (Product # PA5-29589). Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD36 Polyclonal Antibody (Product # PA5-29589) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 µg/mL gentamicin, 1 nM L-glutamin, 500 µM IBMX, 1 µM dexamethasone, 2 µM rosiglitazone and 1 µg/mL insulin.).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD36 Polyclonal Antibody detects CD36 protein at cytosol and weak membrane on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. CD36 Polyclonal Antibody (Product # PA5-29589) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD36 Polyclonal Antibody detects CD36 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. CD36 stained by CD36 Polyclonal Antibody (Product # PA5-29589) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.