Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX114411 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114411, RRID:AB_10619212
- Product name
- Flotillin 2 antibody [N1C1]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references In vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation.
Butko MT, Savas JN, Friedman B, Delahunty C, Ebner F, Yates JR 3rd, Tsien RY
Proceedings of the National Academy of Sciences of the United States of America 2013 Feb 19;110(8):E726-35
Proceedings of the National Academy of Sciences of the United States of America 2013 Feb 19;110(8):E726-35
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE GTX114411 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE Flotillin-2 antibody GTX114411 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flotillin 2 antibody [N1C1] immunoprecipitates Flotillin 2 protein in IP experiments.IP samples: HCT-116 whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of Flotillin 2 protein by 4 £gg Flotillin 2 antibody [N1C1] (GTX114411)10 % SDS-PAGEThe immunoprecipitated Flotillin 2 protein was detected by Flotillin 2 antibody [N1C1] (GTX114411) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Flotillin 2(GTX114411) antibody at 1:100 dilution.