H00125972-M01

antibody from Abnova Corporation

Targeting: CALR3 CRT2, CT93, FLJ25355, MGC26577

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00125972-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00125972-M01, RRID:AB_606011

Product name

CALR3 monoclonal antibody (M01), clone 4E3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant CALR3.

Antigen sequence

VYFQEEFLDGEHWRNRWLQSTNDSRFGHFRLSSGK
FYGHKEKDKGLQTTQNGRFYAISARFKPFSNKGKT
LVIQYTVKHEQKMDCGGGYIKVFPADIDQKNLNGK
SQYYIMFGPDICGFDIKKVHVILHFKNKYHENKKL
IRCKVDGFTHLYTLILRPDLSYDVKIDGQSIESGS
IEYDWNLTSLKKETSPAESKDWEQTKDNKAQDWEK
HFLDASTSKQSDWNGDLDGDWPAPMLQKPPYQDGL
KPEGIHKDVWLHRKMKNTDYLTQYDLSEFENIGAI
GLELWQVRSGTIFDNFLITDDEEYADNFGKATWGE
TKGPEREMDAIQAKEEMKKAREEEEEELLSGKINR
HEHYFNQFHRRNEL


Isotype

IgG

Antibody clone number

4E3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CALR3 expression in transfected 293T cell line by CALR3 monoclonal antibody (M01), clone 4E3.Lane 1: CALR3 transfected lysate(45 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CALR3 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol