AF3264

antibody from Novus Biologicals

Targeting: CDH13 CDHH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AF3264

Provider

Novus Biologicals

Product name

Goat Polyclonal Cadherin-13 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Cadherin-13 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant mouse Cadherin-13 is observed, and less than 10% cross-reactivity with recombinant human (rh) N-Cadherin is observed, and less than 2% cross-reactivity with rhCadherin-8, rhCadherin-11, rhCadherin-17, rhE-Cadherin, rhP-Cadherin, rhVE-Cadherin and rhR-Cadherin is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Pioglitazone strengthen therapeutic effect of adipose-derived regenerative cells against ischemic cardiomyopathy through enhanced expression of adiponectin and modulation of macrophage phenotype.

Mori D, Miyagawa S, Matsuura R, Sougawa N, Fukushima S, Ueno T, Toda K, Kuratani T, Tomita K, Maeda N, Shimomura I, Sawa Y
Cardiovascular diabetology 2019 Mar 22;18(1):39

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Actin cytoskeleton regulates functional anchorage-migration switch during T-cadherin-induced phenotype modulation of vascular smooth muscle cells.

Frismantiene A, Kyriakakis E, Dasen B, Erne P, Resink TJ, Philippova M
Cell adhesion & migration 2018 Jan 2;12(1):69-85

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Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release.

Obata Y, Kita S, Koyama Y, Fukuda S, Takeda H, Takahashi M, Fujishima Y, Nagao H, Masuda S, Tanaka Y, Nakamura Y, Nishizawa H, Funahashi T, Ranscht B, Izumi Y, Bamba T, Fukusaki E, Hanayama R, Shimada S, Maeda N, Shimomura I
JCI insight 2018 Apr 19;3(8)

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The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2.

Fukuda S, Kita S, Obata Y, Fujishima Y, Nagao H, Masuda S, Tanaka Y, Nishizawa H, Funahashi T, Takagi J, Maeda N, Shimomura I
The Journal of biological chemistry 2017 May 12;292(19):7840-7849

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Vagal nerve stimulation modifies neuronal activity and the proteome of excitatory synapses of amygdala/piriform cortex.

Alexander GM, Huang YZ, Soderblom EJ, He XP, Moseley MA, McNamara JO
Journal of neurochemistry 2017 Feb;140(4):629-644

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T-Cadherin Expression in the Epidermis and Adnexal Structures of Normal Skin.

Buechner S, Erne P, Resink TJ
Dermatopathology (Basel, Switzerland) 2016 Sep-Dec;3(4):68-78

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Cross-talk between EGFR and T-cadherin: EGFR activation promotes T-cadherin localization to intercellular contacts.

Kyriakakis E, Maslova K, Frachet A, Ferri N, Contini A, Pfaff D, Erne P, Resink TJ, Philippova M
Cellular signalling 2013 May;25(5):1044-53

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T-cadherin is essential for adiponectin-mediated revascularization.

Parker-Duffen JL, Nakamura K, Silver M, Kikuchi R, Tigges U, Yoshida S, Denzel MS, Ranscht B, Walsh K
The Journal of biological chemistry 2013 Aug 23;288(34):24886-97

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Identification of proteins associating with glycosylphosphatidylinositol- anchored T-cadherin on the surface of vascular endothelial cells: role for Grp78/BiP in T-cadherin-dependent cell survival.

Philippova M, Ivanov D, Joshi MB, Kyriakakis E, Rupp K, Afonyushkin T, Bochkov V, Erne P, Resink TJ
Molecular and cellular biology 2008 Jun;28(12):4004-17

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Cadherin-13 by Western Blot. Western blot shows lysates of human brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cadherin-13 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Cadherin-13 by Simple Western^TM. Simple Western lane view shows lysates of human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Cadherin-13 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cadherin-13 in Human Skin. Cadherin-13 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.